Detailed information of TA system TA169214

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 124046..124310
• Replicon: plasmid pCTX_A3_3
• Accession: NZ_CP059837
• Organism: Escherichia coli strain tcmA_3

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: GQF93_RS26945
• Protein ID: WP_001303307.1
• Coordinates: 124046..124198 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	124248..124310 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQF93_RS26920 (119250)	119250..121418	+	2169	WP_000698360.1	IncI1-type conjugal transfer membrane protein TraY	-
GQF93_RS26925 (121494)	121494..122108	+	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
GQF93_RS26930 (122206)	122206..122415	+	210	WP_001140545.1	hemolysin expression modulator Hha	-
GQF93_RS26935 (122624)	122624..122800	+	177	WP_001054900.1	hypothetical protein	-
- (123285)	123285..123336	+	52	NuclAT_2	-	-
- (123285)	123285..123336	+	52	NuclAT_2	-	-
- (123285)	123285..123336	+	52	NuclAT_2	-	-
- (123285)	123285..123336	+	52	NuclAT_2	-	-
GQF93_RS26940 (123723)	123723..123974	+	252	WP_001291965.1	hypothetical protein	-
GQF93_RS26945 (124046)	124046..124198	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
- (124248)	124248..124310	+	63	NuclAT_0	-	Antitoxin
- (124248)	124248..124310	+	63	NuclAT_0	-	Antitoxin
- (124248)	124248..124310	+	63	NuclAT_0	-	Antitoxin
- (124248)	124248..124310	+	63	NuclAT_0	-	Antitoxin
GQF93_RS26950 (124825)	124825..125604	+	780	WP_275450201.1	protein FinQ	-
- (125671)	125671..125731	+	61	NuclAT_1	-	-
- (125671)	125671..125731	+	61	NuclAT_1	-	-
- (125671)	125671..125731	+	61	NuclAT_1	-	-
- (125671)	125671..125731	+	61	NuclAT_1	-	-
GQF93_RS26955 (125911)	125911..127119	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
GQF93_RS26960 (127138)	127138..128208	+	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-65 / fosA3 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia	-	1..133615	133615

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T169214 WP_001303307.1 NZ_CP059837:c124198-124046 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T169214 NZ_CP076305:c1042207-1041989 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT169214 NZ_CP059837:124248-124310 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin