Detailed information of TA system TA169199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3217977..3218579
• Replicon: chromosome
• Accession: NZ_CP059835
• Organism: Escherichia coli strain tcmA_3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: GQF93_RS15625
• Protein ID: WP_000897305.1
• Coordinates: 3217977..3218288 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GQF93_RS15630
• Protein ID: WP_000356397.1
• Coordinates: 3218289..3218579 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQF93_RS15600 (3213891)	3213891..3214490	+	600	WP_001295269.1	glucose-1-phosphatase	-
GQF93_RS15605 (3214484)	3214484..3215356	+	873	WP_000920762.1	virulence factor BrkB family protein	-
GQF93_RS15610 (3215353)	3215353..3215790	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
GQF93_RS15615 (3215835)	3215835..3216776	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
GQF93_RS15620 (3216840)	3216840..3217748	-	909	WP_001162704.1	alpha/beta hydrolase	-
GQF93_RS15625 (3217977)	3217977..3218288	+	312	WP_000897305.1	hypothetical protein	Toxin
GQF93_RS15630 (3218289)	3218289..3218579	+	291	WP_000356397.1	NadS family protein	Antitoxin
GQF93_RS15635 (3219184)	3219184..3219402	+	219	WP_001295676.1	CopG family transcriptional regulator	-
GQF93_RS15640 (3219621)	3219621..3219863	+	243	WP_001086388.1	protein YiiF	-
GQF93_RS15645 (3220092)	3220092..3221072	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-
GQF93_RS15650 (3221471)	3221471..3222400	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
GQF93_RS15655 (3222397)	3222397..3223032	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3220092..3221072	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T169199 WP_000897305.1 NZ_CP059835:3217977-3218288 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T169199 NZ_CP076301:3710877-3711050 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT169199 WP_000356397.1 NZ_CP059835:3218289-3218579 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT169199 NZ_CP076301:3710553-3710849 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7