Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2413990..2414789 Replicon chromosome
Accession NZ_CP059835
Organism Escherichia coli strain tcmA_3

Toxin (Protein)


Gene name yhaV Uniprot ID A0A6H2GMC5
Locus tag GQF93_RS11760 Protein ID WP_000347279.1
Coordinates 2414325..2414789 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag GQF93_RS11755 Protein ID WP_001307405.1
Coordinates 2413990..2414325 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQF93_RS11740 (2409775) 2409775..2410545 - 771 WP_212487635.1 2-dehydro-3-deoxyglucarate aldolase -
GQF93_RS11745 (2410561) 2410561..2411895 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
GQF93_RS11750 (2412270) 2412270..2413841 + 1572 WP_001273741.1 galactarate dehydratase -
GQF93_RS11755 (2413990) 2413990..2414325 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GQF93_RS11760 (2414325) 2414325..2414789 + 465 WP_000347279.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GQF93_RS11765 (2414844) 2414844..2415653 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
GQF93_RS11770 (2415902) 2415902..2417182 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
GQF93_RS11775 (2417205) 2417205..2417678 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
GQF93_RS11780 (2417689) 2417689..2418468 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
GQF93_RS11785 (2418458) 2418458..2419336 + 879 WP_001380079.1 PTS N-acetylgalactosamine transporter subunit IID -
GQF93_RS11790 (2419354) 2419354..2419788 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2403115..2414789 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17869.24 Da        Isoelectric Point: 9.4947

>T169197 WP_000347279.1 NZ_CP059835:2414325-2414789 [Escherichia coli]
MDFPQRVNGWVLYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESFTQETEENH

Download         Length: 465 bp

>T169197 NZ_CP076301:c3022000-3021893 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT169197 WP_001307405.1 NZ_CP059835:2413990-2414325 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT169197 NZ_CP076301:3022058-3022112 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6H2GMC5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References