Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 214896..215431 Replicon plasmid pA7214_P1
Accession NZ_CP059748
Organism Enterococcus faecium strain A7214

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag HI844_RS14705 Protein ID WP_002314366.1
Coordinates 214896..215168 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag HI844_RS14710 Protein ID WP_002297422.1
Coordinates 215168..215431 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HI844_RS14665 210002..210451 - 450 WP_010729386.1 TlpA family protein disulfide reductase -
HI844_RS14670 210607..211149 - 543 WP_002298088.1 hypothetical protein -
HI844_RS14675 211424..212067 + 644 Protein_229 transposase -
HI844_RS14680 212143..212823 + 681 WP_002331383.1 IS6-like element IS1216 family transposase -
HI844_RS14685 212882..213070 - 189 Protein_231 hypothetical protein -
HI844_RS14690 213071..213928 - 858 WP_002292680.1 AAA family ATPase -
HI844_RS14695 214214..214500 - 287 Protein_233 type II toxin-antitoxin system RelE/ParE family toxin -
HI844_RS14700 214490..214819 - 330 WP_002292678.1 AbrB family transcriptional regulator -
HI844_RS14705 214896..215168 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
HI844_RS14710 215168..215431 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HI844_RS14715 215665..216573 + 909 Protein_237 IS982 family transposase -
HI844_RS14720 216576..217751 - 1176 WP_002336205.1 MFS transporter -
HI844_RS14725 217754..220003 - 2250 WP_002314387.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..228284 228284
- inside IScluster/Tn - - 203333..226032 22699


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T169135 WP_002314366.1 NZ_CP059748:c215168-214896 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T169135 NZ_CP076282:c1340072-1339965 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCATCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT169135 WP_002297422.1 NZ_CP059748:c215431-215168 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT169135 NZ_CP076282:1340125-1340186 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References