Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 216407..216942 Replicon plasmid pA4694_P1
Accession NZ_CP059740
Organism Enterococcus faecium strain A4694

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag HI848_RS14095 Protein ID WP_002314366.1
Coordinates 216407..216679 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag HI848_RS14100 Protein ID WP_002297422.1
Coordinates 216679..216942 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HI848_RS14055 211514..211963 - 450 WP_010729386.1 TlpA family protein disulfide reductase -
HI848_RS14060 212119..212661 - 543 WP_002298088.1 hypothetical protein -
HI848_RS14065 212936..213579 + 644 Protein_230 transposase -
HI848_RS14070 213655..214335 + 681 WP_002331383.1 IS6-like element IS1216 family transposase -
HI848_RS14075 214394..214582 - 189 Protein_232 hypothetical protein -
HI848_RS14080 214583..215440 - 858 WP_002292680.1 AAA family ATPase -
HI848_RS14085 215725..216011 - 287 Protein_234 type II toxin-antitoxin system RelE/ParE family toxin -
HI848_RS14090 216001..216330 - 330 WP_002292678.1 AbrB family transcriptional regulator -
HI848_RS14095 216407..216679 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
HI848_RS14100 216679..216942 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HI848_RS14105 217176..218084 + 909 Protein_238 IS982 family transposase -
HI848_RS14110 218087..219262 - 1176 WP_002336205.1 MFS transporter -
HI848_RS14115 219265..221514 - 2250 WP_002314387.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..229795 229795
- inside IScluster/Tn - - 204845..227543 22698


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T169129 WP_002314366.1 NZ_CP059740:c216679-216407 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T169129 NZ_CP076281:2667-2945 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAACGGTCGTG
TGGAAGGAACCCGGGAACTGGTTATTCACCCCCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
GTCCTGCGCGTGTTGCATACCGCACAGAAGTGGCCATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT169129 WP_002297422.1 NZ_CP059740:c216942-216679 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT169129 NZ_CP076281:2398-2667 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCCGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTTCCGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References