Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4062538..4063232 Replicon chromosome
Accession NZ_CP059723
Organism Escherichia coli strain BUCT2930

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag H1R14_RS21280 Protein ID WP_001263489.1
Coordinates 4062834..4063232 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag H1R14_RS21275 Protein ID WP_000554758.1
Coordinates 4062538..4062831 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1R14_RS21250 4058355..4058822 + 468 WP_000725257.1 flagellar basal body-associated FliL family protein -
H1R14_RS21255 4058842..4059558 + 717 WP_000938723.1 FliA/WhiG family RNA polymerase sigma factor -
H1R14_RS21260 4059571..4060434 + 864 WP_001169518.1 flagellar motor stator protein MotA -
H1R14_RS21265 4060437..4061360 + 924 WP_001232546.1 putative lateral flagellar export/assembly protein LafU -
H1R14_RS21270 4061431..4062486 + 1056 WP_001226164.1 DNA polymerase IV -
H1R14_RS21275 4062538..4062831 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
H1R14_RS21280 4062834..4063232 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
H1R14_RS21285 4063242..4063694 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
H1R14_RS21290 4064012..4064218 + 207 Protein_3816 RtcB family protein -
H1R14_RS21295 4064214..4064735 + 522 Protein_3817 peptide chain release factor H -
H1R14_RS21300 4064792..4066249 - 1458 WP_112929358.1 cytosol nonspecific dipeptidase -
H1R14_RS21305 4066510..4066968 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 4067564..4067644 + 81 NuclAT_11 - -
- 4067564..4067644 + 81 NuclAT_11 - -
- 4067564..4067644 + 81 NuclAT_11 - -
- 4067564..4067644 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T169106 WP_001263489.1 NZ_CP059723:4062834-4063232 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T169106 NZ_CP076278:c45554-45402 [Escherichia coli]
ATGCCACAGCGAACATTTTTAATGATGTTGATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGGCTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT169106 WP_000554758.1 NZ_CP059723:4062538-4062831 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT169106 NZ_CP076278:45604-45666 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References