Detailed information of TA system TA169085

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 19001..19270
• Replicon: plasmid p2buct2930
• Accession: NZ_CP059718
• Organism: Escherichia coli strain BUCT2930

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H1R14_RS00145
• Protein ID: WP_001312861.1
• Coordinates: 19112..19270 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	19001..19066 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1R14_RS00115	14781..15314	+	534	WP_105272431.1	single-stranded DNA-binding protein	-
H1R14_RS00120	15372..15605	+	234	WP_000005998.1	DUF905 family protein	-
H1R14_RS00125	15669..17627	+	1959	WP_105272432.1	ParB/RepB/Spo0J family partition protein	-
H1R14_RS00130	17682..18116	+	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
H1R14_RS00135	18113..18832	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
H1R14_RS00140	18844..19032	-	189	WP_001299721.1	hypothetical protein	-
-	18844..19068	+	225	NuclAT_0	-	-
-	18844..19068	+	225	NuclAT_0	-	-
-	18844..19068	+	225	NuclAT_0	-	-
-	18844..19068	+	225	NuclAT_0	-	-
-	19001..19066	-	66	-	-	Antitoxin
H1R14_RS00145	19112..19270	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
H1R14_RS00150	19508..19885	-	378	Protein_27	hypothetical protein	-
H1R14_RS00155	20185..20481	+	297	WP_001272251.1	hypothetical protein	-
H1R14_RS00160	20592..21413	+	822	WP_001234445.1	DUF945 domain-containing protein	-
H1R14_RS00165	21710..22312	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
H1R14_RS00170	22635..23018	+	384	WP_001354030.1	relaxosome protein TraM	-
H1R14_RS00175	23212..23883	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
H1R14_RS00180	24020..24247	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / oqxA / oqxB / sul2 / aph(3'')-Ib / aph(6)-Id	-	1..73818	73818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T169085 WP_001312861.1 NZ_CP059718:19112-19270 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T169085 NZ_CP076276:c1386233-1386126 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCATCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT169085 NZ_CP059718:c19066-19001 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin