Detailed information of TA system TA169066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 97352..97998
• Replicon: chromosome
• Accession: NZ_CP059711
• Organism: Enterobacter hormaechei strain ZHH

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H2678_RS00480
• Protein ID: WP_038415013.1
• Coordinates: 97648..97998 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H2678_RS00475
• Protein ID: WP_038415012.1
• Coordinates: 97352..97651 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2678_RS00450	92820..93404	-	585	WP_094948322.1	type 1 fimbrial protein	-
H2678_RS00455	93457..94059	-	603	WP_038415009.1	response regulator transcription factor	-
H2678_RS00460	94894..95682	+	789	WP_038415010.1	winged helix-turn-helix domain-containing protein	-
H2678_RS00465	95684..96163	+	480	WP_022646606.1	hypothetical protein	-
H2678_RS00470	96406..97287	+	882	WP_038415011.1	helix-turn-helix domain-containing protein	-
H2678_RS00475	97352..97651	-	300	WP_038415012.1	XRE family transcriptional regulator	Antitoxin
H2678_RS00480	97648..97998	-	351	WP_038415013.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H2678_RS00485	98266..98518	-	253	Protein_96	DNA-binding protein	-
H2678_RS00490	98621..98962	-	342	WP_038415015.1	SymE family type I addiction module toxin	-
H2678_RS00495	99088..99279	+	192	WP_038416963.1	toxin-antitoxin system HicB family antitoxin	-
H2678_RS00500	99285..99911	-	627	WP_051979808.1	hypothetical protein	-
H2678_RS00505	100122..100442	-	321	WP_071605960.1	TA system toxin CbtA family protein	-
H2678_RS00510	100484..100840	-	357	WP_044599779.1	type IV toxin-antitoxin system YeeU family antitoxin	-
H2678_RS00515	100872..101093	-	222	WP_057696015.1	DUF987 family protein	-
H2678_RS00520	101102..101572	-	471	WP_023305179.1	DNA repair protein RadC	-
H2678_RS00525	101642..102463	-	822	WP_044599778.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13521.57 Da
• Isoelectric Point: 8.8448

>T169066 WP_038415013.1 NZ_CP059711:c97998-97648 [Enterobacter hormaechei]
MWTVLFSKVFEQWLLEQEDGLQEKVLADLLNLQNYGPRLPRPYADTVKGSQYKNMKELRIQYAGRPVRAFFAFDPVRQAI
VLCAGDKRNDKTFYEKLIRIADAEFTHHLTSLEAAK

Nucleotide

• Length: 351 bp

>T169066 NZ_CP076274:3437836-3438210 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11057.80 Da
• Isoelectric Point: 6.4695

>AT169066 WP_038415012.1 NZ_CP059711:c97651-97352 [Enterobacter hormaechei]
VKTLNEVIASRSPESQQRIQEMAEEMFLETGLQLLREELQLSQKHVAALMGVSQPAVTQLEQRGNDLKIATLKRYIEAMG
GKLSINVELPTGKQVAFHL

Nucleotide

• Length: 300 bp

>AT169066 NZ_CP076274:3437378-3437746 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAAGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA