Detailed information of TA system TA169065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4680950..4681539
• Replicon: chromosome
• Accession: NZ_CP059710
• Organism: Xanthomonas oryzae pv. oryzicola strain ML5

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A0C5V8F2
• Locus tag: H4J00_RS21290
• Protein ID: WP_024712137.1
• Coordinates: 4680950..4681231 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H4J00_RS21295
• Protein ID: WP_011260746.1
• Coordinates: 4681249..4681539 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4J00_RS21285 (H4J00_21185)	4678594..4680855	+	2262	WP_047339413.1	exodeoxyribonuclease V subunit alpha	-
H4J00_RS21290 (H4J00_21190)	4680950..4681231	+	282	WP_024712137.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H4J00_RS21295 (H4J00_21195)	4681249..4681539	+	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
H4J00_RS21300 (H4J00_21200)	4682452..4683846	+	1395	WP_024712138.1	type III secretion system effector XopQ	-
H4J00_RS21305	4683949..4684136	-	188	Protein_4049	hypothetical protein	-
H4J00_RS21310 (H4J00_21205)	4684297..4684629	-	333	WP_011260743.1	hypothetical protein	-
H4J00_RS21315 (H4J00_21210)	4684884..4685189	+	306	WP_224229802.1	hypothetical protein	-
H4J00_RS21320 (H4J00_21215)	4685339..4685761	+	423	WP_044751863.1	PAAR domain-containing protein	-
H4J00_RS21325 (H4J00_21220)	4685764..4686240	+	477	WP_047339412.1	DUF4123 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11125.91 Da
• Isoelectric Point: 10.5318

>T169065 WP_024712137.1 NZ_CP059710:4680950-4681231 [Xanthomonas oryzae pv. oryzicola]
MIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYNIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T169065 NZ_CP076274:c3283507-3283100 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT169065 WP_011260746.1 NZ_CP059710:4681249-4681539 [Xanthomonas oryzae pv. oryzicola]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT169065 NZ_CP076274:c3283754-3283488 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0C5V8F2