Detailed information of TA system TA169050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4759762..4760351
• Replicon: chromosome
• Accession: NZ_CP059591
• Organism: Xanthomonas oryzae pv. oryzae strain IXO221

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: IXO221_RS22300
• Protein ID: WP_012443754.1
• Coordinates: 4760070..4760351 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IXO221_RS22295
• Protein ID: WP_011260746.1
• Coordinates: 4759762..4760052 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IXO221_RS22270 (IXO221_022230)	4754960..4755286	-	327	WP_237384726.1	hypothetical protein	-
IXO221_RS22275 (IXO221_022235)	4755355..4756389	-	1035	WP_011407587.1	IS630 family transposase	-
IXO221_RS22280 (IXO221_022240)	4756657..4756989	+	333	WP_011260743.1	hypothetical protein	-
IXO221_RS22285	4757149..4757336	+	188	Protein_4256	hypothetical protein	-
IXO221_RS22290 (IXO221_022245)	4757439..4758833	-	1395	WP_075240796.1	type III secretion system effector XopQ	-
IXO221_RS22295 (IXO221_022250)	4759762..4760052	-	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
IXO221_RS22300 (IXO221_022255)	4760070..4760351	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IXO221_RS22305 (IXO221_022260)	4760446..4762704	-	2259	WP_075240794.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T169050 WP_012443754.1 NZ_CP059591:c4760351-4760070 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T169050 NZ_CP076273:c84083-83778 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT169050 WP_011260746.1 NZ_CP059591:c4760052-4759762 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT169050 NZ_CP076273:c84303-84085 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5