Detailed information of TA system TA169043

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2104286..2104875
• Replicon: chromosome
• Accession: NZ_CP059590
• Organism: Xanthomonas oryzae pv. oryzae strain IXO685

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: IXO685_RS10025
• Protein ID: WP_012443754.1
• Coordinates: 2104594..2104875 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IXO685_RS10020
• Protein ID: WP_011260746.1
• Coordinates: 2104286..2104576 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IXO685_RS09990 (IXO685_0009980)	2099571..2100047	-	477	WP_011260741.1	DUF4123 domain-containing protein	-
IXO685_RS09995 (IXO685_0009985)	2100050..2100472	-	423	WP_033013301.1	PAAR domain-containing protein	-
IXO685_RS10000 (IXO685_0009990)	2100600..2100926	-	327	WP_228329596.1	hypothetical protein	-
IXO685_RS10005 (IXO685_0009995)	2101181..2101513	+	333	WP_011260743.1	hypothetical protein	-
IXO685_RS10010	2101673..2101860	+	188	Protein_1908	hypothetical protein	-
IXO685_RS10015 (IXO685_0010000)	2101963..2103357	-	1395	WP_011260745.1	type III secretion system effector XopQ	-
IXO685_RS10020 (IXO685_0010005)	2104286..2104576	-	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
IXO685_RS10025 (IXO685_0010010)	2104594..2104875	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IXO685_RS10030 (IXO685_0010015)	2104970..2107222	-	2253	WP_012443753.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T169043 WP_012443754.1 NZ_CP059590:c2104875-2104594 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T169043 NZ_CP076272:c3912750-3912223 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT169043 WP_011260746.1 NZ_CP059590:c2104576-2104286 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT169043 NZ_CP076272:c3913023-3912757 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5