Detailed information of TA system TA169037

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 336356..336945
• Replicon: chromosome
• Accession: NZ_CP059589
• Organism: Xanthomonas oryzae pv. oryzae strain IXO675

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: IXO675_RS01490
• Protein ID: WP_012443754.1
• Coordinates: 336356..336637 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IXO675_RS01495
• Protein ID: WP_011260746.1
• Coordinates: 336655..336945 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IXO675_RS01485 (IXO675_001495)	334009..336261	+	2253	WP_069960050.1	exodeoxyribonuclease V subunit alpha	-
IXO675_RS01490 (IXO675_001500)	336356..336637	+	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IXO675_RS01495 (IXO675_001505)	336655..336945	+	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
IXO675_RS01500 (IXO675_001510)	337874..339268	+	1395	WP_075242691.1	type III secretion system effector XopQ	-
IXO675_RS01505	339371..339558	-	188	Protein_295	hypothetical protein	-
IXO675_RS01510 (IXO675_001515)	339718..340050	-	333	WP_011260743.1	hypothetical protein	-
IXO675_RS01515 (IXO675_001520)	340305..340631	+	327	WP_228329596.1	hypothetical protein	-
IXO675_RS01520 (IXO675_001525)	340768..341181	+	414	WP_012443758.1	PAAR domain-containing protein	-
IXO675_RS01525 (IXO675_001530)	341184..341660	+	477	WP_011260741.1	DUF4123 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T169037 WP_012443754.1 NZ_CP059589:336356-336637 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T169037 NZ_CP076272:c2307107-2306853 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTTTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT169037 WP_011260746.1 NZ_CP059589:336655-336945 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT169037 NZ_CP076272:c2307355-2307104 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5