Detailed information of TA system TA169025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4759435..4760024
• Replicon: chromosome
• Accession: NZ_CP059586
• Organism: Xanthomonas oryzae pv. oryzae strain IXO639

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: IXO639_RS22210
• Protein ID: WP_012443754.1
• Coordinates: 4759743..4760024 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IXO639_RS22205
• Protein ID: WP_011260746.1
• Coordinates: 4759435..4759725 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IXO639_RS22175 (IXO639_022205)	4754720..4755196	-	477	WP_011260741.1	DUF4123 domain-containing protein	-
IXO639_RS22180 (IXO639_022210)	4755199..4755621	-	423	WP_033013301.1	PAAR domain-containing protein	-
IXO639_RS22185 (IXO639_022215)	4755749..4756075	-	327	WP_237384726.1	hypothetical protein	-
IXO639_RS22190 (IXO639_022220)	4756330..4756662	+	333	WP_011260743.1	hypothetical protein	-
IXO639_RS22195	4756822..4757009	+	188	Protein_4238	hypothetical protein	-
IXO639_RS22200 (IXO639_022225)	4757112..4758506	-	1395	WP_075240796.1	type III secretion system effector XopQ	-
IXO639_RS22205 (IXO639_022230)	4759435..4759725	-	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
IXO639_RS22210 (IXO639_022235)	4759743..4760024	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IXO639_RS22215 (IXO639_022240)	4760119..4762377	-	2259	WP_075240794.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T169025 WP_012443754.1 NZ_CP059586:c4760024-4759743 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T169025 NZ_CP076271:c94072-93767 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT169025 WP_011260746.1 NZ_CP059586:c4759725-4759435 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT169025 NZ_CP076271:c94292-94074 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5