Detailed information of TA system TA169016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4892281..4892870
• Replicon: chromosome
• Accession: NZ_CP059584
• Organism: Xanthomonas oryzae pv. oryzae strain IXO493

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: IXO493_RS23030
• Protein ID: WP_012443754.1
• Coordinates: 4892589..4892870 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IXO493_RS23025
• Protein ID: WP_011260746.1
• Coordinates: 4892281..4892571 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IXO493_RS22995 (IXO493_022895)	4887566..4888042	-	477	WP_011260741.1	DUF4123 domain-containing protein	-
IXO493_RS23000 (IXO493_022900)	4888045..4888458	-	414	WP_012443758.1	PAAR domain-containing protein	-
IXO493_RS23005 (IXO493_022905)	4888595..4888921	-	327	WP_228329596.1	hypothetical protein	-
IXO493_RS23010 (IXO493_022910)	4889176..4889508	+	333	WP_011260743.1	hypothetical protein	-
IXO493_RS23015	4889668..4889855	+	188	Protein_4399	hypothetical protein	-
IXO493_RS23020 (IXO493_022915)	4889958..4891352	-	1395	WP_011260745.1	type III secretion system effector XopQ	-
IXO493_RS23025 (IXO493_022920)	4892281..4892571	-	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
IXO493_RS23030 (IXO493_022925)	4892589..4892870	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IXO493_RS23035 (IXO493_022930)	4892965..4895223	-	2259	WP_075240794.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T169016 WP_012443754.1 NZ_CP059584:c4892870-4892589 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T169016 NZ_CP076270:c3036323-3035988 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAACTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT169016 WP_011260746.1 NZ_CP059584:c4892571-4892281 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT169016 NZ_CP076270:c3036571-3036323 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5