Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4275348..4276078 Replicon chromosome
Accession NZ_CP059572
Organism Actinomadura graeca strain 32-07

Toxin (Protein)


Gene name HigB2 Uniprot ID -
Locus tag AGRA3207_RS18795 Protein ID WP_231336026.1
Coordinates 4275689..4276078 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag AGRA3207_RS18790 Protein ID WP_231336025.1
Coordinates 4275348..4275689 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGRA3207_RS18745 (AGRA3207_003730) 4270716..4270892 + 177 WP_231336016.1 DUF397 domain-containing protein -
AGRA3207_RS18750 (AGRA3207_003731) 4270889..4271074 + 186 WP_231336017.1 DUF397 domain-containing protein -
AGRA3207_RS18755 (AGRA3207_003732) 4271083..4271271 + 189 WP_231336018.1 DUF397 domain-containing protein -
AGRA3207_RS18760 (AGRA3207_003733) 4271281..4271463 + 183 WP_231336019.1 DUF397 domain-containing protein -
AGRA3207_RS18765 (AGRA3207_003734) 4271460..4271636 + 177 WP_231336020.1 DUF397 domain-containing protein -
AGRA3207_RS18770 (AGRA3207_003735) 4271768..4272064 + 297 WP_231336021.1 hypothetical protein -
AGRA3207_RS18775 (AGRA3207_003736) 4272289..4273659 + 1371 WP_231336022.1 S41 family peptidase -
AGRA3207_RS18780 (AGRA3207_003737) 4273800..4274471 + 672 WP_231336023.1 hypothetical protein -
AGRA3207_RS18785 (AGRA3207_003738) 4274584..4275261 + 678 WP_231336024.1 hypothetical protein -
AGRA3207_RS18790 (AGRA3207_003739) 4275348..4275689 - 342 WP_231336025.1 XRE family transcriptional regulator Antitoxin
AGRA3207_RS18795 (AGRA3207_003740) 4275689..4276078 - 390 WP_231336026.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AGRA3207_RS18800 (AGRA3207_003741) 4276152..4277384 + 1233 WP_231336027.1 AAA family ATPase -
AGRA3207_RS18805 (AGRA3207_003742) 4277598..4277924 + 327 WP_231336028.1 helix-turn-helix transcriptional regulator -
AGRA3207_RS18810 (AGRA3207_003743) 4277924..4278739 + 816 WP_231336029.1 hypothetical protein -
AGRA3207_RS18815 (AGRA3207_003744) 4278739..4279050 + 312 WP_231336030.1 hypothetical protein -
AGRA3207_RS18820 (AGRA3207_003745) 4279058..4279582 + 525 WP_231336031.1 hypothetical protein -
AGRA3207_RS18825 (AGRA3207_003746) 4279737..4280162 + 426 WP_231336032.1 hypothetical protein -
AGRA3207_RS18830 (AGRA3207_003747) 4280252..4281010 + 759 WP_231336033.1 NAD(P)H-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4269064..4277384 8320


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(35-91)


Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14215.23 Da        Isoelectric Point: 6.4799

>T168995 WP_231336026.1 NZ_CP059572:c4276078-4275689 [Actinomadura graeca]
VTWKIVLVDEVRDWLHGLRGSDRDTLLSISAAIDVLQLRGPALGRPLVDTLRGSTLPNLKELRPGSLGSSEVRILFAFDP
VRDAILLVGGDKSSDWNGWYKEAIPIAEKRYAAHLEELAEGRGQEKGKS

Download         Length: 390 bp

>T168995 NZ_CP076268:c3183742-3183335 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12415.96 Da        Isoelectric Point: 5.0019

>AT168995 WP_231336025.1 NZ_CP059572:c4275689-4275348 [Actinomadura graeca]
MGVEWEDLRDELLVGPDDKAEVARRRNRLLAEERAHRLAEARRRRQLTQKDIAASMGVTQSRVSAIERGSLGRAELGTLA
AYVAALGGRLEIVADFGDEKLVIGDSDYPESGP

Download         Length: 342 bp

>AT168995 NZ_CP076268:c3183989-3183723 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References