Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4112806..4113506 Replicon chromosome
Accession NZ_CP059572
Organism Actinomadura graeca strain 32-07

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag AGRA3207_RS18015 Protein ID WP_231335842.1
Coordinates 4112806..4113180 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag AGRA3207_RS18020 Protein ID WP_231335843.1
Coordinates 4113177..4113506 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGRA3207_RS18005 (AGRA3207_003585) 4110306..4110740 + 435 WP_231335840.1 thioredoxin -
AGRA3207_RS18010 (AGRA3207_003586) 4110737..4112374 + 1638 WP_231335841.1 FAD-dependent oxidoreductase -
AGRA3207_RS18015 (AGRA3207_003587) 4112806..4113180 + 375 WP_231335842.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AGRA3207_RS18020 (AGRA3207_003588) 4113177..4113506 + 330 WP_231335843.1 XRE family transcriptional regulator Antitoxin
AGRA3207_RS18025 (AGRA3207_003589) 4113879..4114262 - 384 WP_231335844.1 DUF5997 family protein -
AGRA3207_RS18030 (AGRA3207_003590) 4114320..4115003 + 684 WP_231335845.1 LysR family transcriptional regulator substrate-binding protein -
AGRA3207_RS18035 (AGRA3207_003591) 4115257..4115841 - 585 WP_231335846.1 hypothetical protein -
AGRA3207_RS18040 (AGRA3207_003592) 4116385..4116696 + 312 WP_231335847.1 GntR family transcriptional regulator -
AGRA3207_RS18045 (AGRA3207_003593) 4116693..4117193 + 501 WP_231335848.1 hypothetical protein -
AGRA3207_RS18050 (AGRA3207_003594) 4117377..4117769 - 393 WP_231335849.1 ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(22-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(43-96)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14071.01 Da        Isoelectric Point: 4.7975

>T168994 WP_231335842.1 NZ_CP059572:4112806-4113180 [Actinomadura graeca]
VSWDVILLEPVESWFLKLCESDVDTAALIEQAIDRLAVVGPTLGRPLVDTLEHSELRNLKELRPGSRGRSEIRMLFVFDP
DRAAIFLVAGDKAGQWSRWYDQAIPLAEARYSEYRAARDKEAGR

Download         Length: 375 bp

>T168994 NZ_CP076268:c3047390-3047055 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11928.44 Da        Isoelectric Point: 7.0743

>AT168994 WP_231335843.1 NZ_CP059572:4113177-4113506 [Actinomadura graeca]
MSTGRRWQDIKAEAHHRNPELADPQRQAQARAELDAYVAGHHLKELRKAIGKTQAEIAQILGVSQSRVSQIENGDLEAME
LETLRAYATALGGHLDITVSVGPHSIKVA

Download         Length: 330 bp

>AT168994 NZ_CP076268:c3047638-3047390 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References