Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4082802..4083502 Replicon chromosome
Accession NZ_CP059572
Organism Actinomadura graeca strain 32-07

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag AGRA3207_RS17870 Protein ID WP_231335809.1
Coordinates 4082802..4083176 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag AGRA3207_RS17875 Protein ID WP_231335810.1
Coordinates 4083173..4083502 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGRA3207_RS17865 (AGRA3207_003556) 4078843..4081278 - 2436 WP_231335808.1 class I SAM-dependent DNA methyltransferase -
AGRA3207_RS17870 (AGRA3207_003558) 4082802..4083176 + 375 WP_231335809.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AGRA3207_RS17875 (AGRA3207_003559) 4083173..4083502 + 330 WP_231335810.1 XRE family transcriptional regulator Antitoxin
AGRA3207_RS17880 (AGRA3207_003560) 4084534..4085307 + 774 WP_231335811.1 cyclase family protein -
AGRA3207_RS17885 (AGRA3207_003561) 4085335..4086162 + 828 WP_231335812.1 3-keto-5-aminohexanoate cleavage protein -
AGRA3207_RS17890 (AGRA3207_003562) 4086280..4086687 - 408 WP_231335813.1 gamma-glutamylcyclotransferase -
AGRA3207_RS17895 (AGRA3207_003563) 4086863..4088242 - 1380 WP_231335815.1 dihydropyrimidinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(22-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(43-96)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14069.98 Da        Isoelectric Point: 4.5931

>T168993 WP_231335809.1 NZ_CP059572:4082802-4083176 [Actinomadura graeca]
VPWDVILLEPVESWFLKLCESETDTAALIEQAIDRLAEVGPTLGRPLVDTLEHSELRNLKELRPGSRGRSEIRMLFVFDP
DRAAIFLVAGDKAGQWSRWYDEAIPLAEARYAEYRAAKDKEGGR

Download         Length: 375 bp

>T168993 NZ_CP076268:c2738472-2738074 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11934.45 Da        Isoelectric Point: 8.6427

>AT168993 WP_231335810.1 NZ_CP059572:4083173-4083502 [Actinomadura graeca]
MSTGRRWQDIKAEAHRRNPELANPERQAQARTELDAYVAGHHLKELRKAVGKTQAEVAQILGVSQSRVSQIENGNLEAME
LETLRAYATALGGHVDITVSVGPHSIKVA

Download         Length: 330 bp

>AT168993 NZ_CP076268:c2738699-2738472 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References