Detailed information of TA system TA168963

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 5551556..5552055
• Replicon: chromosome
• Accession: NZ_CP059556
• Organism: Saccharopolyspora gregorii strain BM8-3

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: H1226_RS24250
• Protein ID: WP_258342983.1
• Coordinates: 5551804..5552055 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: H1226_RS24245
• Protein ID: WP_258342982.1
• Coordinates: 5551556..5551807 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1226_RS24220	5547293..5547472	+	180	WP_224956023.1	hypothetical protein	-
H1226_RS24225	5547809..5548288	+	480	WP_258342975.1	CAP domain-containing protein	-
H1226_RS24230	5548339..5549169	-	831	WP_258342978.1	carbohydrate ABC transporter permease	-
H1226_RS24235	5549166..5550062	-	897	WP_258342980.1	sugar ABC transporter permease	-
H1226_RS24240	5550062..5551333	-	1272	WP_258342981.1	ABC transporter substrate-binding protein	-
H1226_RS24245	5551556..5551807	+	252	WP_258342982.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
H1226_RS24250	5551804..5552055	+	252	WP_258342983.1	Txe/YoeB family addiction module toxin	Toxin
H1226_RS24255	5552138..5552665	-	528	WP_224956030.1	magnesium transporter	-
H1226_RS24260	5552745..5553755	-	1011	WP_258342985.1	CPBP family intramembrane metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 10065.39 Da
• Isoelectric Point: 8.9194

>T168963 WP_258342983.1 NZ_CP059556:5551804-5552055 [Saccharopolyspora gregorii]
MKLVWDENAWADYVWWQTQDRKVLKRINTLLKDVERNGNEGIGKPEPLKHGFWSRRITDEHRLVYRVHEDQIQIAACRYH
YGS

Nucleotide

• Length: 252 bp

>T168963 NZ_CP076264:c1492866-1492672 [Escherichia coli]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGGGATTTCAGGATATTACCG
TGTGAAATGCAATGGATGCGAATCACGATCTGGTTATGGAGGAAGTGAAAAAGCTGCCCTCGAAAGATGGAATAAACGAA
CCATTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9316.46 Da
• Isoelectric Point: 4.6882

>AT168963 WP_258342982.1 NZ_CP059556:5551556-5551807 [Saccharopolyspora gregorii]
MKTMSYSESRAKYAETLNSVVDDREEVVITRAGHEAVVMVALDDYESLKETAYLLKSPENARRLLASIERLEHGGGTTRD
LVE

Nucleotide

• Length: 252 bp

>AT168963 NZ_CP076264:1492495-1492673 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCACATTCTGATTTGGTTAGTTTTGTTTTTGAGTATCTTGTCCAGTTGGTAGGCGAACCGCCTTCCTTTT
CAATAGTGGCAGTAATTTT