Detailed information of TA system TA168953

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): bsrG-as-bsrG/-
• Location: 1865368..1865665
• Replicon: chromosome
• Accession: NZ_CP059497
• Organism: Bacillus velezensis strain JSRB 08

Toxin (Protein)

• Gene name: bsrG
• Uniprot ID: L8EAY0
• Locus tag: H2N97_RS09035
• Protein ID: WP_009967548.1
• Coordinates: 1865549..1865665 (-)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	as-bsrG
Locus tag	-
Coordinates	1865368..1865554 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2N97_RS09010	1860676..1861659	-	984	WP_182077776.1	thermonuclease family protein	-
H2N97_RS09015	1861831..1863546	+	1716	WP_182077777.1	ribonuclease YeeF family protein	-
H2N97_RS09020	1863555..1864052	+	498	WP_019712870.1	SMI1/KNR4 family protein	-
H2N97_RS09025	1864116..1864694	+	579	WP_182077778.1	SMI1/KNR4 family protein	-
H2N97_RS09030	1864730..1865263	+	534	WP_019712872.1	GNAT family N-acetyltransferase	-
-	1865368..1865554	+	187	NuclAT_0	-	Antitoxin
-	1865368..1865554	+	187	NuclAT_0	-	Antitoxin
-	1865368..1865554	+	187	NuclAT_0	-	Antitoxin
-	1865368..1865554	+	187	NuclAT_0	-	Antitoxin
H2N97_RS09035	1865549..1865665	-	117	WP_009967548.1	type I toxin-antitoxin system toxin BsrG	Toxin
H2N97_RS09040	1865905..1866108	+	204	WP_041481757.1	hypothetical protein	-
H2N97_RS09045	1866238..1867011	+	774	WP_182077779.1	sporulation protein YunB	-
H2N97_RS09050	1867239..1867571	+	333	WP_065180792.1	YolD-like family protein	-
H2N97_RS09055	1867564..1868814	+	1251	WP_062623501.1	UV damage repair protein UvrX	-
H2N97_RS09060	1868978..1870138	-	1161	WP_079891309.1	tetratricopeptide repeat protein	-
H2N97_RS09065	1870290..1870550	-	261	WP_041482578.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1842315..1996289	153974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (1-38)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4336.39 Da
• Isoelectric Point: 10.1022

>T168953 WP_009967548.1 NZ_CP059497:c1865665-1865549 [Bacillus velezensis]
MTVYESLMIMINFGGLILNTVLLIFNIMMIVTSSQKKK

Nucleotide

• Length: 117 bp

>T168953 NZ_CP076262:4717303-4717476 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Nucleotide

• Length: 187 bp

>AT168953 NZ_CP059497:1865368-1865554 [Bacillus velezensis]
AATGATACAAATAATTATTTATTGCATTTTGATTTGTGGAAATGCATAAAATAAAAAAAGACCTGGGGTGCGCTAACACC
CACTGGTCATTGTTTGTACAAAAAGCTGCCCATCAAAGGGCTTGCTCGTTGTCAAATCTGCGTAGGCCTAACCCTTCAGG
TGTCCAAACTCAAGGGAAGGTCTATTT

Structures

Toxin

• AlphaFold DB: A0A7Z7EVU9

Antitoxin