Detailed information of TA system TA168929

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Gp49-HTH_37
• Location: 966618..967237
• Replicon: chromosome
• Accession: NZ_CP059488
• Organism: Acidithiobacillus ferrivorans strain XJFY6S-08

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H2515_RS04975
• Protein ID: WP_198661046.1
• Coordinates: 966911..967237 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H2515_RS04970
• Protein ID: WP_035192166.1
• Coordinates: 966618..966914 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2515_RS04950	961979..963325	-	1347	WP_198661042.1	sensor domain-containing diguanylate cyclase	-
H2515_RS04955	964049..964693	-	645	WP_198661043.1	AAA family ATPase	-
H2515_RS04960	964618..965112	-	495	WP_198661044.1	hypothetical protein	-
H2515_RS04965	965271..966308	-	1038	WP_198661045.1	hypothetical protein	-
H2515_RS04970	966618..966914	-	297	WP_035192166.1	helix-turn-helix domain-containing protein	Antitoxin
H2515_RS04975	966911..967237	-	327	WP_198661046.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H2515_RS04980	967269..968585	-	1317	WP_198661411.1	integrase family protein	-
H2515_RS04985	969238..969504	-	267	WP_198661047.1	hypothetical protein	-
H2515_RS04990	969525..969749	-	225	WP_065412568.1	hypothetical protein	-
H2515_RS04995	969798..970076	+	279	WP_198661048.1	hypothetical protein	-
H2515_RS05000	970087..971052	+	966	WP_198661049.1	DUF932 domain-containing protein	-
H2515_RS05005	971079..971366	+	288	WP_198661050.1	YqaJ viral recombinase family protein	-
H2515_RS05010	971341..971946	+	606	WP_198661051.1	YqaJ viral recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	964618..989559	24941

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-82)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12370.58 Da
• Isoelectric Point: 10.3374

>T168929 WP_198661046.1 NZ_CP059488:c967237-966911 [Acidithiobacillus ferrivorans]
MNWIVSTLDQRVDAELASIPKPILARLVRILEMIEQVGLEHVHEPHIKHLEAKLWEIRAKGPDGIARAIYVTASGKRVVI
LHAFVKKTQKTPKQILEIARQRAREVEE

Nucleotide

• Length: 327 bp

>T168929 NZ_CP076259:4551224-4551397 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10614.21 Da
• Isoelectric Point: 10.2144

>AT168929 WP_035192166.1 NZ_CP059488:c966914-966618 [Acidithiobacillus ferrivorans]
MTRLTELKKEWMQDADFRAEYVALEEEFAIAHALIQARIKAGLSQAEVARRMGTTQSVVARLEGGGSLPSLRTIQRYASA
TGARAVVKIVPGQSTPVG

Nucleotide

• Length: 297 bp

>AT168929 NZ_CP076259:4550900-4551196 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG