Detailed information of TA system TA168925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 157054..157629
• Replicon: chromosome
• Accession: NZ_CP059488
• Organism: Acidithiobacillus ferrivorans strain XJFY6S-08

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H2515_RS00670
• Protein ID: WP_014027637.1
• Coordinates: 157054..157278 (+)
• Length: 75 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1E7XSU4
• Locus tag: H2515_RS00675
• Protein ID: WP_071182739.1
• Coordinates: 157330..157629 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2515_RS00655	153126..153806	-	681	WP_065414210.1	hydrogenase	-
H2515_RS00660	153816..154757	-	942	WP_065414209.1	NADH-quinone oxidoreductase subunit H	-
H2515_RS00665	154904..156565	-	1662	WP_071182738.1	glycosyltransferase family 39 protein	-
H2515_RS00670	157054..157278	+	225	WP_014027637.1	hypothetical protein	Toxin
H2515_RS00675	157330..157629	+	300	WP_071182739.1	helix-turn-helix domain-containing protein	Antitoxin
H2515_RS00680	157772..158776	-	1005	WP_190277962.1	glycosyltransferase family 4 protein	-
H2515_RS00685	159565..160587	-	1023	WP_071182741.1	glycosyltransferase	-
H2515_RS00690	160611..161672	-	1062	WP_071182742.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-72)

Sequences

Toxin

Protein

• Length: 75 a.a.
• Molecular weight: 8150.55 Da
• Isoelectric Point: 10.3475

>T168925 WP_014027637.1 NZ_CP059488:157054-157278 [Acidithiobacillus ferrivorans]
MVIVESPVFTKAIVAILDDEGYRAMQNALVENPALGVVIPHGGGLRKVRWGVEGRGKRGGIRVIYYWWTGKGQI

Nucleotide

• Length: 225 bp

>T168925 NZ_CP076259:3460767-3461231 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11065.63 Da
• Isoelectric Point: 8.9086

>AT168925 WP_071182739.1 NZ_CP059488:157330-157629 [Acidithiobacillus ferrivorans]
MDEQGFDELLDSVRWMGRHMRGEDDNGRTTRIPDPDVKAIRETTGLSQSQFALMIGVKLKTLQNWEQKRVRPAGPARALL
QIVAVDPHAAIRALHGHAA

Nucleotide

• Length: 300 bp

>AT168925 NZ_CP076259:3460432-3460767 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A1E7XSU4