Detailed information of TA system TA168920

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4931678..4932408
• Replicon: chromosome
• Accession: NZ_CP059481
• Organism: Enterobacter kobei strain IB2020

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2W0GI63
• Locus tag: H1R18_RS24740
• Protein ID: WP_023331686.1
• Coordinates: 4932094..4932408 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H1R18_RS24735
• Protein ID: WP_127353113.1
• Coordinates: 4931678..4932097 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1R18_RS24710	4927166..4928671	-	1506	WP_045134486.1	signal transduction histidine-protein kinase/phosphatase UhpB	-
H1R18_RS24715	4928668..4929261	-	594	WP_014881931.1	transcriptional regulator UhpA	-
H1R18_RS24720	4929344..4929631	-	288	WP_010426528.1	acetolactate synthase small subunit	-
H1R18_RS24725	4929635..4931323	-	1689	WP_014881930.1	acetolactate synthase large subunit	-
H1R18_RS24730	4931430..4931528	-	99	WP_023331688.1	ilvB operon leader peptide IvbL	-
H1R18_RS24735	4931678..4932097	-	420	WP_127353113.1	helix-turn-helix domain-containing protein	Antitoxin
H1R18_RS24740	4932094..4932408	-	315	WP_023331686.1	type II toxin-antitoxin system HigB family toxin	Toxin
H1R18_RS24745	4932756..4933976	+	1221	WP_000343760.1	ISL3-like element ISKox3 family transposase	-
H1R18_RS24750	4934220..4934309	+	90	WP_023331685.1	type I toxin-antitoxin system toxin TisB	-
H1R18_RS24755	4934374..4934820	+	447	WP_014881927.1	GNAT family N-acetyltransferase	-
H1R18_RS24760	4934883..4935716	+	834	WP_050862199.1	DMT family transporter	-
H1R18_RS24765	4935892..4937076	+	1185	WP_045269345.1	multidrug efflux MFS transporter EmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4932756..4933976	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12441.59 Da
• Isoelectric Point: 10.2825

>T168920 WP_023331686.1 NZ_CP059481:c4932408-4932094 [Enterobacter kobei]
MHLISMKAILDAVIQFPQHREELLFLGRTIEKSHCTTPVALRKIFPTLDNLKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T168920 NZ_CP076259:c2261910-2261656 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15538.14 Da
• Isoelectric Point: 5.2250

>AT168920 WP_127353113.1 NZ_CP059481:c4932097-4931678 [Enterobacter kobei]
MMIVADAMKATHALVAAVPLLGENPNEKDYQDALELVEYLLMNEPGSPLLDIVCARIRRYEANRPEIVALRQEMESVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT168920 NZ_CP076259:c2262158-2261907 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A2W0GI63