Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3720871..3721520 Replicon chromosome
Accession NZ_CP059481
Organism Enterobacter kobei strain IB2020

Toxin (Protein)


Gene name higB Uniprot ID A0A2W0G579
Locus tag H1R18_RS18940 Protein ID WP_045281668.1
Coordinates 3720871..3721233 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2W0GCS7
Locus tag H1R18_RS18945 Protein ID WP_014882863.1
Coordinates 3721221..3721520 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1R18_RS18920 3716207..3717178 - 972 WP_014882868.1 LacI family transcriptional regulator -
H1R18_RS18925 3717254..3718396 - 1143 WP_014882867.1 Gfo/Idh/MocA family oxidoreductase -
H1R18_RS18930 3718389..3719414 - 1026 WP_110820402.1 sugar phosphate isomerase/epimerase -
H1R18_RS18935 3719430..3720665 - 1236 WP_023332295.1 MFS transporter -
H1R18_RS18940 3720871..3721233 + 363 WP_045281668.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H1R18_RS18945 3721221..3721520 + 300 WP_014882863.1 helix-turn-helix transcriptional regulator Antitoxin
H1R18_RS18950 3721631..3723019 - 1389 WP_014882862.1 phenylalanine transporter -
H1R18_RS18955 3723122..3725095 - 1974 WP_050862672.1 PhoX family phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-80)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13910.87 Da        Isoelectric Point: 5.6482

>T168917 WP_045281668.1 NZ_CP059481:3720871-3721233 [Enterobacter kobei]
MWNVETTDIFDQWYFEQTTALKEDVLAMMHILAEFGPTLGRPYVDTVKASAYANMKELRIQHAGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKRFYNEMITLADVEYSRHLADKEAIWQR

Download         Length: 363 bp

>T168917 NZ_CP076259:1621660-1621836 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10860.61 Da        Isoelectric Point: 9.8627

>AT168917 WP_014882863.1 NZ_CP059481:3721221-3721520 [Enterobacter kobei]
MATLKELMAQQSAESQERIAAKVDVIRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GEMSIDVTLPNGKRIAFQI

Download         Length: 300 bp

>AT168917 NZ_CP076259:1621882-1622298 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W0G579

Antitoxin

Source ID Structure
AlphaFold DB A0A2W0GCS7

References