Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2070803..2071400 Replicon chromosome
Accession NZ_CP059468
Organism Klebsiella pneumoniae strain BA25665

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag HBJ46_RS09840 Protein ID WP_004142563.1
Coordinates 2070803..2071120 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag HBJ46_RS09845 Protein ID WP_004142561.1
Coordinates 2071113..2071400 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HBJ46_RS09825 (2066506) 2066506..2067933 - 1428 WP_004176980.1 MFS transporter -
HBJ46_RS09830 (2068042) 2068042..2068908 + 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
HBJ46_RS09835 (2069575) 2069575..2070618 + 1044 WP_004178895.1 DUF2157 domain-containing protein -
HBJ46_RS09840 (2070803) 2070803..2071120 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HBJ46_RS09845 (2071113) 2071113..2071400 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
HBJ46_RS09850 (2071668) 2071668..2072318 - 651 Protein_1909 oxygen-insensitive NAD(P)H nitroreductase -
HBJ46_RS09855 (2072438) 2072438..2072806 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
HBJ46_RS09860 (2072806) 2072806..2073387 - 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
HBJ46_RS09865 (2073567) 2073567..2074682 + 1116 WP_020316605.1 MBL fold metallo-hydrolase -
HBJ46_RS09870 (2074713) 2074713..2075054 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
HBJ46_RS09875 (2075072) 2075072..2075320 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T168879 WP_004142563.1 NZ_CP059468:2070803-2071120 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T168879 NZ_CP076251:c1131854-1131603 [Xanthomonas translucens pv. phlei]
ATGGACACCATCACCTATAGCGCTGCACGCGCCGCCCTCGCGGACACCATGGAGCGCGTCGTCAACAACCACGAGCCGGT
GATCATCACCCGCAGCCGCGAGCAATCCGTGGTCATGCTGTCGCTTGAGGACTACAAGGCGATGGAGGAAACCGCCTACC
TGCTCCGCAGTCCGAAGAGCGCGCAGCGCCTGCTGGAATCCATCGCCCAGCTCGAGGCCGGTCGCGGCAAGGCACGGGAC
CTGGCCGAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT168879 WP_004142561.1 NZ_CP059468:2071113-2071400 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT168879 NZ_CP076251:c1131606-1131349 [Xanthomonas translucens pv. phlei]
GTGACGCTTCAGTTCTCGGACAATGCTTGGGAGGACTACCTCTACTGGCAGCAGACCGACAAGAAGATGCTCAAGCGCAT
CAATGACCTGATCAAAGCCATCCAACACGATCCCTTCCAGGGCGTCGGCAAGCCAGAACCGCTGCGCCATGCCTTGGCCG
GCTATTGGTCGCGGCGCATCAACGACGAACACCGCATCGTGTACAAGGTCGAGGCGGGCGTCCTGCTGATCGCGCAGGTG
CGCTACCACTACGTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References