Detailed information of TA system TA168870

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3735844..3736375
• Replicon: chromosome
• Accession: NZ_CP059467
• Organism: Aromatoleum bremense strain PbN1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: pbN1_RS17555
• Protein ID: WP_244857008.1
• Coordinates: 3735844..3736071 (+)
• Length: 76 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: pbN1_RS17560
• Protein ID: WP_169204319.1
• Coordinates: 3736064..3736375 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
pbN1_RS20995 (pbN1_35620)	3731104..3731766	+	663	WP_280516161.1	WecB/TagA/CpsF family glycosyltransferase	-
pbN1_RS17535 (pbN1_35630)	3731858..3732298	+	441	WP_169204273.1	hypothetical protein	-
pbN1_RS17540 (pbN1_35640)	3732355..3733347	+	993	WP_169204272.1	ParB N-terminal domain-containing protein	-
pbN1_RS17545 (pbN1_35650)	3733253..3734641	+	1389	WP_169204271.1	IS21 family transposase	-
pbN1_RS17550 (pbN1_35660)	3734628..3735422	+	795	WP_210147564.1	IS21-like element helper ATPase IstB	-
pbN1_RS17555 (pbN1_35670)	3735844..3736071	+	228	WP_244857008.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
pbN1_RS17560 (pbN1_35680)	3736064..3736375	+	312	WP_169204319.1	helix-turn-helix transcriptional regulator	Antitoxin
pbN1_RS17565 (pbN1_35690)	3736342..3736713	+	372	WP_244857009.1	type II toxin-antitoxin system RelE/ParE family toxin	-
pbN1_RS17570 (pbN1_35700)	3736861..3738078	+	1218	WP_169204317.1	IS256 family transposase	-
pbN1_RS17575 (pbN1_35710)	3738091..3738390	+	300	WP_169204245.1	DNA-binding transcriptional regulator	-
pbN1_RS17580 (pbN1_35720)	3738442..3738666	+	225	WP_244857010.1	hypothetical protein	-
pbN1_RS17585 (pbN1_35730)	3738699..3738932	+	234	WP_169204243.1	addiction module protein	-
pbN1_RS17590 (pbN1_35740)	3738933..3739226	+	294	WP_169204242.1	type II toxin-antitoxin system RelE/ParE family toxin	-
pbN1_RS17595 (pbN1_35750)	3739313..3739720	+	408	WP_210147565.1	NAD-dependent epimerase/dehydratase family protein	-
pbN1_RS17600 (pbN1_35770)	3739814..3740509	+	696	WP_244857272.1	glycosyltransferase	-
pbN1_RS17605 (pbN1_35780)	3740506..3740643	+	138	WP_169204240.1	hypothetical protein	-
pbN1_RS17610 (pbN1_35790)	3740662..3741048	+	387	WP_169204239.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3721371..3740643	19272
	-	flank	IS/Tn	-	-	3736861..3738078	1217

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (1-70)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-98)

Sequences

Toxin

Protein

• Length: 76 a.a.
• Molecular weight: 8618.02 Da
• Isoelectric Point: 9.8534

>T168870 WP_244857008.1 NZ_CP059467:3735844-3736071 [Aromatoleum bremense]
MEEHGADLRLPHSRAMGDGLFELRCKGEEGIGRVFYCTLVGRQIVILHSFIKKTQETPDRELKTARKRLKEVKHG

Nucleotide

• Length: 228 bp

>T168870 NZ_CP076249:c4419099-4418965 [Xanthomonas translucens pv. hordei]
ATGAAGCGTACTTTCGCGTGGATGCTGCTGGCGATGTTCTCGGTGGGCCTGCTGTCGGGCTGCAACACGGTTGCCGGTGC
CGGCAAGGACGTGAAGAGCGCCGGCGAGAAGGTCGAAGACGCCGCCAAGAACTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11544.28 Da
• Isoelectric Point: 10.2576

>AT168870 WP_169204319.1 NZ_CP059467:3736064-3736375 [Aromatoleum bremense]
MDREHFKPVRLDTKAELARAMQRPGFKAAWDGLEEEYAALNELLQARKQAGLTQEEVAARMGTTKSAVSRLEASIRSESH
SPSFATLRKYAHACGKRLVIKMV

Nucleotide

• Length: 312 bp

>AT168870 NZ_CP076249:c4419350-4419198 [Xanthomonas translucens pv. hordei]
ATGAAGCGACTGTTCACCTTGACGATGCTGACCCTGTTTTGTGCAGGCATGCTGACCGGCTGCAACACCATGGCCGGCGC
CGGTAAGGACATGCAGAAGGCCGGCGATAAGGTGGAAACCAAGGCCGACGACTGTAAAGACGGCAAGTGCTGA