Detailed information of TA system TA168761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1795219..1795879
• Replicon: chromosome
• Accession: NZ_CP059391
• Organism: Klebsiella africana strain FF1003

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H1X61_RS08710
• Protein ID: WP_203033937.1
• Coordinates: 1795526..1795879 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A7U2ZUJ7
• Locus tag: H1X61_RS08705
• Protein ID: WP_061153979.1
• Coordinates: 1795219..1795521 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1X61_RS08670	1790483..1791454	+	972	WP_136032940.1	sensor domain-containing diguanylate cyclase	-
H1X61_RS08675	1791451..1792554	-	1104	WP_203033935.1	phosphotransferase	-
H1X61_RS08680	1792696..1792782	+	87	Protein_1698	type II toxin-antitoxin system PemK/MazF family toxin	-
H1X61_RS08690	1793104..1794540	+	1437	WP_203033936.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
H1X61_RS08700	1794866..1795015	-	150	Protein_1700	antitoxin	-
H1X61_RS08705	1795219..1795521	-	303	WP_061153979.1	XRE family transcriptional regulator	Antitoxin
H1X61_RS08710	1795526..1795879	-	354	WP_203033937.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H1X61_RS08715	1796292..1796750	+	459	WP_136032098.1	hypothetical protein	-
H1X61_RS08720	1796839..1798293	-	1455	WP_203033938.1	AMP nucleosidase	-
H1X61_RS08725	1798424..1798669	-	246	WP_136032102.1	signal transduction protein PmrD	-
H1X61_RS08730	1798940..1800562	+	1623	WP_136032104.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (29-89)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13554.54 Da
• Isoelectric Point: 10.1729

>T168761 WP_203033937.1 NZ_CP059391:c1795879-1795526 [Klebsiella africana]
VWTIKTTDMFDRWFTSLNDIDRASVLAALLVLREKGPGLSRPYADTIRGSRYSNMKELRVQSRGDPIRAFFPFDPARTGI
VLCAGNKVGNEKRFYHEMLAVADREFTNWLNRLKEKE

Nucleotide

• Length: 354 bp

>T168761 NZ_CP076237:c457242-456757 [Escherichia coli O157:H7]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11135.94 Da
• Isoelectric Point: 5.1976

>AT168761 WP_061153979.1 NZ_CP059391:c1795521-1795219 [Klebsiella africana]
MGRTLEQLLIDEKPDVVAEAQAIAKDILLNIHLAELREKVQKTQVEMAQDLGIKQPTVAGMEKPGRDLKLSTLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT168761 NZ_CP076237:c457511-457233 [Escherichia coli O157:H7]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U2ZUJ7