Detailed information of TA system TA168589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 164551..165136
• Replicon: plasmid pAC1633-1
• Accession: NZ_CP059301
• Organism: Acinetobacter baumannii strain AC1633

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: H0529_RS21850
• Protein ID: WP_000897307.1
• Coordinates: 164551..164871 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H0529_RS21855
• Protein ID: WP_000369781.1
• Coordinates: 164864..165136 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0529_RS21820	160594..161346	+	753	Protein_155	IS1-like element ISAba3 family transposase	-
H0529_RS21825	161474..162301	+	828	WP_000073658.1	AraC family transcriptional regulator	-
H0529_RS21830	162351..162956	+	606	WP_000447788.1	LysE family translocator	-
H0529_RS21835	163006..163320	-	315	WP_004728120.1	BrnA antitoxin family protein	-
H0529_RS21840	163307..163594	-	288	WP_000438825.1	BrnT family toxin	-
H0529_RS21845	163866..164396	-	531	WP_000172759.1	hypothetical protein	-
H0529_RS21850	164551..164871	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H0529_RS21855	164864..165136	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
H0529_RS21860	165629..167104	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
H0529_RS21865	167160..168044	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
H0529_RS21870	168234..168845	-	612	WP_015060246.1	recombinase family protein	-
H0529_RS21875	169101..170063	+	963	WP_033917576.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / sul2 / blaNDM-1 / aph(3'')-Ib / aph(6)-Id / blaOXA-58 / msr(E) / mph(E)	-	1..174292	174292
	-	inside	IScluster/Tn	aph(3'')-Ib / aph(6)-Id / blaOXA-58 / msr(E) / mph(E)	-	149082..170063	20981

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T168589 WP_000897307.1 NZ_CP059301:164551-164871 [Acinetobacter baumannii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T168589 NZ_CP076123:2700170-2700388 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT168589 WP_000369781.1 NZ_CP059301:164864-165136 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT168589 NZ_CP076123:2699770-2700144 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3