Detailed information of TA system TA168576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 647607..648205
• Replicon: chromosome
• Accession: NZ_CP059298
• Organism: Providencia rettgeri strain P01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I1YFL9
• Locus tag: H0904_RS02845
• Protein ID: WP_001896384.1
• Coordinates: 647882..648205 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H0904_RS02840
• Protein ID: WP_000058216.1
• Coordinates: 647607..647885 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0904_RS02825	644569..646389	+	1821	WP_181489237.1	conjugative transfer system coupling protein TraD	-
H0904_RS02830	646399..646959	+	561	WP_181489239.1	conjugative transfer protein	-
H0904_RS02835	646946..647581	+	636	WP_181489241.1	DUF4400 domain-containing protein	-
H0904_RS02840	647607..647885	-	279	WP_000058216.1	helix-turn-helix domain-containing protein	Antitoxin
H0904_RS02845	647882..648205	-	324	WP_001896384.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H0904_RS02850	648386..648667	+	282	WP_000433891.1	type IV conjugative transfer system protein TraL	-
H0904_RS02855	648664..649290	+	627	WP_159262613.1	pilus assembly protein	-
H0904_RS02860	649274..650170	+	897	WP_159262630.1	type-F conjugative transfer system secretin TraK	-
H0904_RS02865	650173..651462	+	1290	WP_159262611.1	TraB/VirB10 family protein	-
H0904_RS02870	651537..652109	+	573	WP_148039504.1	type IV conjugative transfer system lipoprotein TraV	-
H0904_RS02875	652106..652492	+	387	WP_032469918.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaCTX-M-3 / mph(E) / msr(E)	-	565857..824232	258375

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12281.33 Da
• Isoelectric Point: 9.7658

>T168576 WP_001896384.1 NZ_CP059298:c648205-647882 [Providencia rettgeri]
MSWKIDFYDGVEDQILDMPPKIQARMIKLLELMEKHGANLGPPHTESMGDGLFEIRAKAQEGIGRGLFCYLKGKHIYVLH
AFVKKSQKTPKNELNLARDRQKEVQKS

Nucleotide

• Length: 324 bp

>T168576 NZ_CP076122:c1352316-1352008 [Ralstonia solanacearum]
ATGCTGACGATCCGCACGACCGAAATCTTCGACGACTGGTTTTGCAGCCTCCGCGACAAGGCTGCGCAACGCCGCATCCA
GGTGCGCATCGACCGCCTGCAGATGGGCCACCCAGGCAACATGAAAGCGGTCCGTGACGGCATCCGTGAGCTGCGGATCG
ATCATGGGCCGGGCGACCGCTTGTACTTCGTGCGGCATGGCGTGGTCCTGATCGTACTGCTCTGCGGCGGCGACAAGTCG
ACGCAGGAAACCGATATCCGTCGCGCAATTGACCTGTCCCGCCGTCTCGACGTGGACGACATGGAATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10517.07 Da
• Isoelectric Point: 9.9393

>AT168576 WP_000058216.1 NZ_CP059298:c647885-647607 [Providencia rettgeri]
MSLQKLKQRALANAEVKNEYDKLESEFSFIDQLLTMRTKAGLTQEQVAERMHTQKSNVSRLEKGNANPSWSTLLKYAHAC
GFELTLKPQKMS

Nucleotide

• Length: 279 bp

>AT168576 NZ_CP076122:c1352011-1351706 [Ralstonia solanacearum]
ATGACGATGGCCCAACGCACCATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTCCGGACCGAAGCGGACATCGCCGC
CTACCTCGACGCCTGCCTGGAAGAAGCCGGAGATGACCCCGCCTTCATCACGCATGCGCTGGGCGTGGTCGCGCGCTCGC
GCGGCATGACGCAGCTCGCCCGCGAGACAGGGATGACCCGCGAAGGGCTGTACAAGGCGCTTTCCGAGGGCGGCAATCCG
AGCTTTGCGACCGTGCTGAAGGTCATCCGGGCGCTCGGCATCCGGCTGCACGCCGTGCCGACCTGA

Structures

Toxin

• AlphaFold DB: A0A244CN87