Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3609422..3610116 Replicon chromosome
Accession NZ_CP059288
Organism Escherichia coli strain 1506

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag H0481_RS17670 Protein ID WP_001263493.1
Coordinates 3609422..3609820 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag H0481_RS17675 Protein ID WP_000554757.1
Coordinates 3609823..3610116 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0481_RS17640 3604981..3605439 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
H0481_RS17645 3605700..3607157 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
H0481_RS17650 3607214..3607735 - 522 Protein_3453 peptide chain release factor H -
H0481_RS17655 3607734..3607937 - 204 Protein_3454 RNA ligase RtcB family protein -
H0481_RS17660 3608273..3608970 + 698 Protein_3455 IS1 family transposase -
H0481_RS17665 3608981..3609412 - 432 WP_181463901.1 GNAT family N-acetyltransferase -
H0481_RS17670 3609422..3609820 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
H0481_RS17675 3609823..3610116 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
H0481_RS17680 3610168..3611220 - 1053 WP_000691315.1 DNA polymerase IV -
H0481_RS17685 3611282..3612826 + 1545 WP_181463902.1 recombinase family protein -
H0481_RS17695 3614379..3615087 + 709 Protein_3462 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - yagV/ecpE 3544133..3621068 76935
- inside IScluster/Tn - - 3608467..3616620 8153


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T168540 WP_001263493.1 NZ_CP059288:c3609820-3609422 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T168540 NZ_CP076050:1481896-1482180 [Cupriavidus metallidurans]
ATGATCCGCTCGCTCTCCGACAAGACGACGGCCGCCATCTATTGCGGCCAGTCCGTTCACACACTGCCCGCTCACATTCA
GGCCGATGCGCTGCGCAAGCTCGCGATCCTCGACGCTGCGTCGTCTCTTCCCGATATGGTCGCGCCGCCCGCAAACCGGC
TGGAACCCGTTCTCGGCAAGCGCCGCGGACACTGGCGCGTGCGCATTCAAGGTCCGTGGCATCTCTGCTTTCGCTTCATC
AACGGCGAAGCGTGGGACGTCGGCATCGCCGAATACGAGGAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT168540 WP_000554757.1 NZ_CP059288:c3610116-3609823 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT168540 NZ_CP076050:1482190-1482537 [Cupriavidus metallidurans]
ATGACAATCAAGCGCGAAGACTTGCGGCGCGCGGATTTTCGCAGCATTGCCACCGGCCGGCCGATCCCGCCTGCACACCC
CGGCGAGGTCCTGGAAACCGAGTTCATGGCGCCAAACGAACTGTCGGCAGAAGCACTGGCGGTGGCCTTGCATATGGCGC
CGCAACGAATTCACGATATCGTGCGACGTCAGCGAGCTATATCGCCAGACACCGCCCTGAGGCTGGCCCGCTATTTCGGC
ACTAGTGCGGAATTCTGGGCGGGCCTCCAGGCCGCGTATGCACTGCGGCTGGCGCGCGCGGCTGCAGGGCCGATCATCGA
GCGCGACATCCAGCCGCTGACGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB S1EXB8


Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References