Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1747169..1747671 Replicon chromosome
Accession NZ_CP059288
Organism Escherichia coli strain 1506

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H0481_RS08240 Protein ID WP_000767829.1
Coordinates 1747417..1747671 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H0481_RS08235 Protein ID WP_001259255.1
Coordinates 1747169..1747420 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0481_RS08210 1742347..1743414 - 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H0481_RS08215 1743414..1744484 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
H0481_RS08220 1744481..1745785 - 1305 WP_004991367.1 histidinol dehydrogenase -
H0481_RS08225 1745791..1746690 - 900 WP_000131756.1 ATP phosphoribosyltransferase -
H0481_RS08230 1746836..1746886 - 51 WP_001364200.1 his operon leader peptide -
H0481_RS08235 1747169..1747420 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H0481_RS08240 1747417..1747671 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H0481_RS08245 1747754..1748578 + 825 WP_000754758.1 SDR family oxidoreductase -
H0481_RS08250 1748624..1749553 + 930 WP_181464052.1 LysR family transcriptional regulator -
H0481_RS08255 1749768..1749830 + 63 WP_010723108.1 membrane protein YoeI -
H0481_RS08260 1749820..1751178 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H0481_RS08265 1751357..1752415 + 1059 WP_000492310.1 transport protein YeeE -
H0481_RS08270 1752429..1752656 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T168529 WP_000767829.1 NZ_CP059288:1747417-1747671 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T168529 NZ_CP076044:2926-3075 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCTACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT168529 WP_001259255.1 NZ_CP059288:1747169-1747420 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT168529 NZ_CP076044:c2882-2821 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References