Detailed information of TA system TA168523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 582922..583703
• Replicon: chromosome
• Accession: NZ_CP059288
• Organism: Escherichia coli strain 1506

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: H0481_RS02810
• Protein ID: WP_113417943.1
• Coordinates: 582922..583368 (-)
• Length: 149 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: H0481_RS02815
• Protein ID: WP_001307405.1
• Coordinates: 583368..583703 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0481_RS02785	578358..579236	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
H0481_RS02790	579226..580005	-	780	WP_000406209.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
H0481_RS02795	580016..580489	-	474	WP_001336162.1	PTS N-acetylgalactosamine transporter subunit IIB	-
H0481_RS02800	580512..581792	-	1281	WP_000681920.1	tagatose-bisphosphate aldolase subunit KbaZ	-
H0481_RS02805	582040..582849	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
H0481_RS02810	582922..583368	-	447	WP_113417943.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
H0481_RS02815	583368..583703	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
H0481_RS02820	583852..585423	-	1572	WP_001273753.1	galactarate dehydratase	-
H0481_RS02825	585798..587132	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
H0481_RS02830	587148..587918	+	771	WP_001058209.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-147)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 149 a.a.
• Molecular weight: 17109.55 Da
• Isoelectric Point: 10.0993

>T168523 WP_113417943.1 NZ_CP059288:c583368-582922 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTR

Nucleotide

• Length: 447 bp

>T168523 NZ_CP076043:c2950922-2950749 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT168523 WP_001307405.1 NZ_CP059288:c583703-583368 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT168523 NZ_CP076043:c2951246-2950950 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0XVC7