Detailed information of TA system TA168518

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 73731..74157
• Replicon: plasmid pSY3626_124k
• Accession: NZ_CP059285
• Organism: Escherichia coli strain SY3626_hybrid

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H0155_RS23955
• Protein ID: WP_001312861.1
• Coordinates: 73731..73889 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	73933..74157 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0155_RS23930	69100..69789	-	690	WP_039002187.1	conjugal transfer transcriptional regulator TraJ	-
H0155_RS23935	69976..70359	-	384	WP_001151524.1	relaxosome protein TraM	-
H0155_RS23940	70680..71282	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
H0155_RS23945	71579..72400	-	822	WP_072795862.1	DUF945 domain-containing protein	-
H0155_RS23950	72522..72809	-	288	WP_000107535.1	hypothetical protein	-
H0155_RS23955	73731..73889	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	73933..74157	-	225	NuclAT_0	-	Antitoxin
-	73933..74157	-	225	NuclAT_0	-	Antitoxin
-	73933..74157	-	225	NuclAT_0	-	Antitoxin
-	73933..74157	-	225	NuclAT_0	-	Antitoxin
H0155_RS23960	73969..74157	+	189	WP_001299721.1	hypothetical protein	-
H0155_RS23965	74169..74888	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
H0155_RS23970	74885..75319	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
H0155_RS23975	75374..77332	-	1959	WP_039000998.1	ParB/RepB/Spo0J family partition protein	-
H0155_RS23980	77397..77630	-	234	WP_000005987.1	DUF905 family protein	-
H0155_RS23985	77687..78226	-	540	WP_032332913.1	single-stranded DNA-binding protein	-
H0155_RS23990	78252..78458	-	207	WP_000275853.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(M) / ant(3'')-Ia / aac(3)-IId / lnu(F) / blaTEM-1B / sul3 / aph(3')-Ia / sul2 / floR	-	1..124445	124445

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T168518 WP_001312861.1 NZ_CP059285:c73889-73731 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T168518 NZ_CP076043:1553923-1554030 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT168518 NZ_CP059285:c74157-73933 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin