168513

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4159566..4160063 Replicon chromosome
Accession NZ_CP059283
Organism Escherichia coli strain SY3626_hybrid

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H0155_RS20365 Protein ID WP_001173343.1
Coordinates 4159566..4159739 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H0155_RS20370 Protein ID WP_000398619.1
Coordinates 4159767..4160063 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0155_RS20355 4156236..4157693 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
H0155_RS20360 4157930..4159447 + 1518 WP_001295074.1 lysine--tRNA ligase -
H0155_RS20365 4159566..4159739 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H0155_RS20370 4159767..4160063 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H0155_RS20375 4160290..4160562 - 273 WP_000405647.1 N-acetyltransferase -
H0155_RS20380 4160574..4160804 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
H0155_RS20385 4160985..4162616 + 1632 WP_001565376.1 sensor histidine kinase -
H0155_RS20390 4162613..4163332 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4150712..4160063 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T168513 WP_001173343.1 NZ_CP059283:c4159739-4159566 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T168513 NZ_CP076043:898129-898232 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT168513 WP_000398619.1 NZ_CP059283:c4160063-4159767 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT168513 NZ_CP076043:c898270-897999 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References