Detailed information of TA system TA168491

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 59867..60196
• Replicon: plasmid pC9
• Accession: NZ_CP059282
• Organism: Escherichia coli strain C9

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H0272_RS24395
• Protein ID: WP_001312861.1
• Coordinates: 60038..60196 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	59867..59994 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0272_RS24375	55148..57106	+	1959	WP_042045562.1	ParB/RepB/Spo0J family partition protein	-
H0272_RS24380	57161..57595	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
H0272_RS24385	57592..58311	+	720	WP_033550421.1	plasmid SOS inhibition protein A	-
-	58323..58425	+	103	NuclAT_1	-	-
-	58323..58425	+	103	NuclAT_1	-	-
-	58323..58425	+	103	NuclAT_1	-	-
-	58323..58425	+	103	NuclAT_1	-	-
H0272_RS24390	58471..59840	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	59867..59994	+	128	NuclAT_0	-	Antitoxin
-	59867..59994	+	128	NuclAT_0	-	Antitoxin
-	59867..59994	+	128	NuclAT_0	-	Antitoxin
-	59867..59994	+	128	NuclAT_0	-	Antitoxin
H0272_RS24395	60038..60196	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
H0272_RS24400	60639..60974	+	336	WP_013023876.1	hypothetical protein	-
H0272_RS24405	60887..61093	+	207	WP_000275859.1	hypothetical protein	-
H0272_RS24410	61118..61405	+	288	WP_000107535.1	hypothetical protein	-
H0272_RS24415	61524..62345	+	822	WP_001234469.1	DUF945 domain-containing protein	-
H0272_RS24420	62642..63244	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
H0272_RS24425	63574..63957	+	384	WP_001151566.1	relaxosome protein TraM	-
H0272_RS24430	64091..64768	+	678	WP_001348626.1	PAS domain-containing protein	-
H0272_RS24435	64856..65083	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / mph(A) / sul1 / qacE / aadA5 / dfrA17	senB	1..140350	140350

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T168491 WP_001312861.1 NZ_CP059282:60038-60196 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T168491 NZ_CP076027:c2164863-2164654 [Staphylococcus sp. MZ7]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Nucleotide

• Length: 128 bp

>AT168491 NZ_CP059282:59867-59994 [Escherichia coli]
CGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGAAGATAGCCCCGTAGTAA
GTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin