Detailed information of TA system TA168456

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 22185..22514
• Replicon: plasmid pC7
• Accession: NZ_CP059280
• Organism: Escherichia coli strain C7

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H0271_RS24210
• Protein ID: WP_001312861.1
• Coordinates: 22356..22514 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	22185..22312 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0271_RS24190	17466..19424	+	1959	WP_042045562.1	ParB/RepB/Spo0J family partition protein	-
H0271_RS24195	19479..19913	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
H0271_RS24200	19910..20629	+	720	WP_033550421.1	plasmid SOS inhibition protein A	-
-	20641..20743	+	103	NuclAT_1	-	-
-	20641..20743	+	103	NuclAT_1	-	-
-	20641..20743	+	103	NuclAT_1	-	-
-	20641..20743	+	103	NuclAT_1	-	-
H0271_RS24205	20789..22158	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	22185..22312	+	128	NuclAT_0	-	Antitoxin
-	22185..22312	+	128	NuclAT_0	-	Antitoxin
-	22185..22312	+	128	NuclAT_0	-	Antitoxin
-	22185..22312	+	128	NuclAT_0	-	Antitoxin
H0271_RS24210	22356..22514	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
H0271_RS24215	22957..23292	+	336	WP_013023876.1	hypothetical protein	-
H0271_RS24220	23205..23411	+	207	WP_000275859.1	hypothetical protein	-
H0271_RS24225	23436..23723	+	288	WP_000107535.1	hypothetical protein	-
H0271_RS24230	23842..24663	+	822	WP_001234469.1	DUF945 domain-containing protein	-
H0271_RS24235	24960..25562	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
H0271_RS24240	25892..26275	+	384	WP_001151566.1	relaxosome protein TraM	-
H0271_RS24245	26409..27086	+	678	WP_001348626.1	PAS domain-containing protein	-
H0271_RS24250	27174..27401	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / mph(A) / sul1 / dfrA17 / aadA5	senB	1..141169	141169

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T168456 WP_001312861.1 NZ_CP059280:22356-22514 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T168456 NZ_CP076008:842801-843211 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Nucleotide

• Length: 128 bp

>AT168456 NZ_CP059280:22185-22312 [Escherichia coli]
CGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGAAGATAGCCCCGTAGTAA
GTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin