Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4517112..4517911 Replicon chromosome
Accession NZ_CP059279
Organism Escherichia coli strain C7

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag H0271_RS21730 Protein ID WP_000347251.1
Coordinates 4517447..4517911 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag H0271_RS21725 Protein ID WP_001296435.1
Coordinates 4517112..4517447 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0271_RS21710 4512897..4513667 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
H0271_RS21715 4513683..4515017 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
H0271_RS21720 4515392..4516963 + 1572 WP_001273763.1 galactarate dehydratase -
H0271_RS21725 4517112..4517447 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H0271_RS21730 4517447..4517911 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H0271_RS21735 4517966..4518775 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H0271_RS21740 4519024..4520304 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
H0271_RS21745 4520327..4520800 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H0271_RS21750 4520811..4521590 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
H0271_RS21755 4521580..4522458 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
H0271_RS21760 4522476..4522910 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T168453 WP_000347251.1 NZ_CP059279:4517447-4517911 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T168453 NZ_CP075935:26843-27433 [Clostridium perfringens]
ATGATCGATATTTCTATATTAGATAAATTAAGTTACAAAAGTAATTTAAATAAAAGTCTCAAAAATACACTAAGAAAATT
TAATAAAGAAGATATTATGACTGAACTTTTGGATATAAGAGGATTTTATGAAAAAAATCAATTATTGACAACCATTAAAT
TTGATTATAGAATAAAATCATTTCAAAGTTCTTTGTTGAAATATGACAAATACTTTCCAAGTACTGAATTGAAAAAATGT
TTTAATGACTTGCTTGGATTTAGAATTGTTATTGATAATTATAATGAATTAGATAATATTAGCTTAGAAAATTTTAGAAT
TGCAGATATGCGTAATGGAAAAGCTAATGATGATGGATATAGAGGTATACATTTATATTATCAAAAATCTAATAGACATT
ATCCTATTGAGCTGCAAATAAATACAGTAAAAGATAGAATTTTCAATGATTGGTTACATCAAGAATTATATAAATATGTT
TCTAATAATAATATAGGTATCGAACTTAGAAAAAAATATGATGATGGTATTATAACATCATTGGAAGATTTTAAAGGAGA
ATTAAAAAAGCATGTGTTATATAATATCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT168453 WP_001296435.1 NZ_CP059279:4517112-4517447 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT168453 NZ_CP075935:27414-27638 [Clostridium perfringens]
ATGTGTTATATAATATCTAAAAAATTCAATGAACTAGGTTGTTATGCTCTTAAAAGTGAATTAGGGGATAAATTAGCTTC
ATTATCAAGATATCTTACTTTAGCAACACTAGATAAAAATGTTCAAATAGTAACAATAAGTGATCCTAATGTTTATAAGG
AATATGAACCATACACTTTTGTTATTTCAGAAAAAGAATTTATAAATAAAACTTTAGAATTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References