Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3131926..3132428 Replicon chromosome
Accession NZ_CP059279
Organism Escherichia coli strain C7

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag H0271_RS15310 Protein ID WP_000767822.1
Coordinates 3131926..3132180 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H0271_RS15315 Protein ID WP_001259255.1
Coordinates 3132177..3132428 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0271_RS15290 3128419..3129777 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
H0271_RS15295 3129767..3129829 - 63 WP_010723108.1 membrane protein YoeI -
H0271_RS15300 3130044..3130973 - 930 WP_000803366.1 LysR family transcriptional regulator -
H0271_RS15305 3131019..3131843 - 825 WP_000754737.1 SDR family oxidoreductase -
H0271_RS15310 3131926..3132180 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H0271_RS15315 3132177..3132428 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H0271_RS15320 3132711..3132761 + 51 WP_001364200.1 his operon leader peptide -
H0271_RS15325 3132907..3133806 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
H0271_RS15330 3133812..3135116 + 1305 WP_001546022.1 histidinol dehydrogenase -
H0271_RS15335 3135113..3136183 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
H0271_RS15340 3136183..3137250 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T168449 WP_000767822.1 NZ_CP059279:c3132180-3131926 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T168449 NZ_CP075896:3154387-3154668 [Streptomyces sp. MG62]
ATGGGGTACGTCACGCGCTTCACCCCGCACGCCCAGCGGGACATGCTCAAGATCCCGCGCCCTGATGCCCTGCGCATCCT
GTACCGCTTGACCGAACTCCAGAAGGCGATGGATGCGGGGGAGACGGCGGCCTTTGATGTGAAGGCACTCCGAGGGCACA
ACGCCCGGTGGCGACTCAGGGTCGGTGACTACCGGGTCGTCTACACCGTCGAGGACGGTCGAGTCGTCGTGTGGGTCCTG
ACCGTGGGCAACCGGCGGGAGGTCTACCGTCAAGTTCCCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT168449 WP_001259255.1 NZ_CP059279:c3132428-3132177 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT168449 NZ_CP075896:3154100-3154381 [Streptomyces sp. MG62]
ATGAGTGAGCCGGTTGTGGAATCCATGGCCGAGGTCCGCAGTCACCTCGCCGACGTGATCGACCGCGCCCGCCGCGACAA
GACACCGACGATCATCACCCGGCGCGGCAAGCGGGAAGCCGTCGTGATCGACATCGAGGAGTACCAGCGGCTGCGCCAGC
TCGCCGAGGACGCCGAAGAGGCATGGCTCAACCGGCTGGCCGACGAAGCCGAGTCCGAAGGCACCGACGGGTCGGTCTCA
CTCGAAGAGATGGCCGCCCTGCTCCGCGACCAGCAGCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References