Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1540797..1541476 Replicon chromosome
Accession NZ_CP059279
Organism Escherichia coli strain C7

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag H0271_RS07350 Protein ID WP_000057523.1
Coordinates 1540797..1541099 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag H0271_RS07355 Protein ID WP_000806442.1
Coordinates 1541135..1541476 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0271_RS07325 1536173..1537825 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
H0271_RS07330 1537863..1538366 - 504 WP_000667000.1 hypothetical protein -
H0271_RS07335 1538363..1539163 - 801 WP_000439798.1 hypothetical protein -
H0271_RS07340 1539187..1539666 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
H0271_RS07345 1539870..1540664 - 795 WP_000365147.1 TraB/GumN family protein -
H0271_RS07350 1540797..1541099 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H0271_RS07355 1541135..1541476 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
H0271_RS07360 1541534..1544038 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
H0271_RS07365 1544300..1545232 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T168433 WP_000057523.1 NZ_CP059279:1540797-1541099 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T168433 NZ_CP075885:c4247339-4246017 [Klebsiella quasipneumoniae]
ATGGCGACGCTCACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCCAACGGGGCGCACAGTTTCCG
CTATGATGACGAGTGGTTGCGCAGCCCGCGCGCCAGGCCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATCACCG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCACAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAACAGCCGTTCGATCTGCTGGCCGAGGTCGGCCGGGATAGCGTAGGGGCGGTGACTCTGCTGCCGCCGGG
AGAAGAGGCTCATCTTGCAGCGTTGCGCTGGCAGACGCTGGATGAGGCGCAACTGACCGCATTGCTGACCGCCTACCAGT
CGGATATTCCGCTCGGGATGGTGACCGGCCAGGACGATTTTCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACCGCGCTG
CTGAGAATGGGCGAGCACTGGTGTATTCCGCAGGGCACCACCCCGACCACCCATATCATTAAACTGCCCATTGGCGAGAT
AAAACAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAATATCTGTGCCTGGCGCTGGCCAGGGCGTTGG
GCCTGGCGGTGCCGGAGGCGGAGATTATCACCACCCCGCGGATCCGTGCGCTGGCGGTGACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGGCGCGTGCTGCTGCGGCTGCCGCAGGAGGATCTGTGTCAGGCATTCGGCATCCCGTCGTCGATGAAATA
CGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCCAGTGAAGCCCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTGTTCCAGTGGCTGACCGGGGCAACGGACGGGCATGCGAAGAATTTCTCGCTCTTTCTGCTG
CCCGGCGGCAGCTATCGGCTCACGCCGTTCTACGACATTATTTCGGCATTTCCGGTGCTGGGAGGGACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAACGCCACCCGAGGGCGGAAAACGGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACCGCCAAAGCGGTTAACTTTCCCCGGGATCAGATGCTGGCGATCCTTGACGAGTTTGCCGACCGCGTGCCG
CAGGCCATCGAAAGCGCCCGCCGGACGCTGCCGGTGGACTTTTCCGCCCACGTCTGGCGGGCGATCACGGAGAACATGCT
GAAACTGCACGCCCGCCTGCGGCAAGGGCTGCAGGCGCGATAG

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT168433 WP_000806442.1 NZ_CP059279:1541135-1541476 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT168433 NZ_CP075885:c4247605-4247339 [Klebsiella quasipneumoniae]
ATGCATTCCCCGCAGATATACAGTCCCGTTCAGCTGGCCAATTACCTGAAACTGCTGCGCCAGCAAAATCACTGGACTCA
GGAGGCGCTGGCCAGCCGGATCGGCATTAAACAGGCGACGCTGTCCAATTTTGAAAATCACCCGGACAAAACCACCTTGA
CCACCCTGTTCAAGATCCTCCAGTCGCTGGGGGTGGTGATGACCCTGAACGCCAAACCGGCCGAGACGGCGACGGCCCCC
GATAACACCACAGATCTGGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References