Detailed information of TA system TA168424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2841875..2842551
• Replicon: chromosome
• Accession: NZ_CP059254
• Organism: Streptomyces albidoflavus strain J1074/R2

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: D6AZC1
• Locus tag: XNRR2_RS12280
• Protein ID: WP_003949888.1
• Coordinates: 2842189..2842551 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: XNRR2_RS12275
• Protein ID: WP_015507386.1
• Coordinates: 2841875..2842192 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XNRR2_RS12250	2837161..2838222	+	1062	WP_008404846.1	hypothetical protein	-
XNRR2_RS12255	2838438..2838968	+	531	WP_003949893.1	toxin-antitoxin system HicB family antitoxin	-
XNRR2_RS12260	2839070..2839939	+	870	WP_003949892.1	DUF4097 family beta strand repeat protein	-
XNRR2_RS12265	2840043..2841017	+	975	WP_003949891.1	daunorubicin resistance protein DrrA family ABC transporter ATP-binding protein	-
XNRR2_RS12270	2841014..2841796	+	783	WP_003949890.1	ABC transporter permease	-
XNRR2_RS12275	2841875..2842192	-	318	WP_015507386.1	helix-turn-helix domain-containing protein	Antitoxin
XNRR2_RS12280	2842189..2842551	-	363	WP_003949888.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
XNRR2_RS12285	2842550..2844247	+	1698	WP_003949889.1	hypothetical protein	-
XNRR2_RS12290	2844337..2844579	-	243	WP_015507388.1	hypothetical protein	-
XNRR2_RS12295	2844883..2845737	+	855	WP_015507389.1	STAS domain-containing protein	-
XNRR2_RS12300	2845734..2846114	+	381	WP_003949885.1	STAS domain-containing protein	-
XNRR2_RS12305	2846114..2846530	+	417	WP_003949884.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13472.34 Da
• Isoelectric Point: 4.7680

>T168424 WP_003949888.1 NZ_CP059254:c2842551-2842189 [Streptomyces albidoflavus]
MDGEWQIFLVDEVRVWLASLDGAAHARVVQALDVLAEEGPALGRPLVDTLRGSAVANLKELRPGTVRILFAFDPWRSSIL
LVAGDKSGQRTEWYQEAIPLAEQRYALYLKEREREEGGRP

Nucleotide

• Length: 363 bp

>T168424 NZ_CP075881:c5936180-5935923 [Klebsiella michiganensis]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCACATCCGCGATGCATC
AGGCCGCGTCACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTTAAAAAAC
TCAAAACCAAAAAGCTGATTAAGTCCGTCAACGGACAGCCCTACCGCATCAATACCACCGGGCTGAATAACGTCCGCGCA
CAGCTGGACAATCGCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11572.07 Da
• Isoelectric Point: 9.8236

>AT168424 WP_015507386.1 NZ_CP059254:c2842192-2841875 [Streptomyces albidoflavus]
MTSYTRWQDIRPEHVARAGGEEAVRAGKEELLAQVTGRKLAELRRARGLTQQEVADRMGVSKGRISQIERGHLSGQEVLA
RFATALGGRLHQAIYFDDGDIATIA

Nucleotide

• Length: 318 bp

>AT168424 NZ_CP075881:c5935912-5935361 [Klebsiella michiganensis]
ATGACAAACCACAGCTTCACTTTTCATGTCGCCTCTGAAGGCGATACCGATGACATTCTCGACGTCGAAACCCGGGCTTT
CGGCTACAGCAAAGAGGCCAGGCTGGTGGCCGATTTGCTCAACGATGAGAGCGCCTGCCCGACGTTATCGCTGCTGGCCA
GGCATAACGGAGAGGCGGTTGGGCATATTTTATTCACCCGGGCGACGTTCAAAGGGGAGCCGGACTCGCCGATGATGCAC
ATCCTTGCTCCCCTGGCGGTGGTGCCGGAATACCAGGGCGTTGGCGTTGGCGGAGGGCTTATCCGGCACGGCATTGAGCA
GTTGAAAGCAATGGGAAGCCAGATGGTATTTGTTCTGGGCCACGCCGCTTACTACCCCAGGCTCGGGTTTGAGCCATGCG
CGGGAGATAAAGGCTATCCGGCGCCTTATCCGATTCCCGAGGCGCATAAAGCCTGCTGGATGCTGCAGCCGCTTTCGTCT
CAGCCGCTGGGACGAACGGGCCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Structures

Toxin

• AlphaFold DB: A0A7Y6SI38