Detailed information of TA system TA168408

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2282919..2283511
• Replicon: chromosome
• Accession: NZ_CP059162
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain YS05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H0I86_RS10620
• Protein ID: WP_180925816.1
• Coordinates: 2283233..2283511 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: H0I86_RS10615
• Protein ID: WP_180924967.1
• Coordinates: 2282919..2283224 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0I86_RS10600	2281055..2281393	+	339	WP_180924964.1	phage tail protein	-
H0I86_RS10605	2281403..2282152	+	750	WP_180924965.1	phage minor tail protein L	-
H0I86_RS10610	2282155..2282922	+	768	WP_180924966.1	C40 family peptidase	-
H0I86_RS10615	2282919..2283224	-	306	WP_180924967.1	HigA family addiction module antidote protein	Antitoxin
H0I86_RS10620	2283233..2283511	-	279	WP_180925816.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H0I86_RS10625	2283650..2284072	+	423	WP_180924968.1	hypothetical protein	-
H0I86_RS10630	2284125..2284727	+	603	WP_180924969.1	tail assembly protein	-
H0I86_RS10635	2284785..2288243	+	3459	WP_180924970.1	phage tail protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2245238..2323092	77854

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10585.01 Da
• Isoelectric Point: 7.8255

>T168408 WP_180925816.1 NZ_CP059162:c2283511-2283233 [Pseudomonas chlororaphis subsp. aurantiaca]
MIISFRCGDTETLFTTGSSRKWRAILPVAQRKLTMLDQAIALQDLRSPPGNRLEALQGNRQGQYSIRINDQYRICFTWDA
QGPENVEIVDYH

Nucleotide

• Length: 279 bp

>T168408 NZ_CP075852:4685993-4686247 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11313.99 Da
• Isoelectric Point: 6.2193

>AT168408 WP_180924967.1 NZ_CP059162:c2283224-2282919 [Pseudomonas chlororaphis subsp. aurantiaca]
VFNNGMRPVHPGEILREEFLLPMDLTPTALARMLHVSPPTVNEIVREKRGITADVALRLAACLNTSAQFWTNLQSAYDLR
RAEIDHGADILRQVERAPEPA

Nucleotide

• Length: 306 bp

>AT168408 NZ_CP075852:4685745-4685996 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA