Detailed information of TA system TA168407

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 2257449..2258104
• Replicon: chromosome
• Accession: NZ_CP059162
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain YS05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7D6EBU8
• Locus tag: H0I86_RS10425
• Protein ID: WP_062823026.1
• Coordinates: 2257449..2257793 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H0I86_RS10430
• Protein ID: WP_124304706.1
• Coordinates: 2257790..2258104 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0I86_RS10385	2252793..2252873	+	81	WP_180925812.1	hypothetical protein	-
H0I86_RS10390	2253535..2253771	+	237	WP_180924933.1	hypothetical protein	-
H0I86_RS10395	2253829..2254682	+	854	WP_180924934.1	IS5 family transposase	-
H0I86_RS10400	2254649..2255305	+	657	WP_180924935.1	hypothetical protein	-
H0I86_RS10405	2255401..2255838	-	438	WP_180924936.1	helix-turn-helix domain-containing protein	-
H0I86_RS10410	2255984..2256145	+	162	WP_016704884.1	Arc family DNA-binding protein	-
H0I86_RS10415	2256126..2256524	+	399	WP_029527292.1	DUF3077 domain-containing protein	-
H0I86_RS10420	2256521..2257315	+	795	Protein_2044	phage antirepressor KilAC domain-containing protein	-
H0I86_RS10425	2257449..2257793	+	345	WP_062823026.1	hypothetical protein	Toxin
H0I86_RS10430	2257790..2258104	+	315	WP_124304706.1	transcriptional regulator	Antitoxin
H0I86_RS10435	2258158..2258583	-	426	WP_029527289.1	hypothetical protein	-
H0I86_RS10440	2258682..2258927	+	246	WP_180924937.1	helix-turn-helix domain-containing protein	-
H0I86_RS10445	2259052..2259474	+	423	WP_180924938.1	Rha family transcriptional regulator	-
H0I86_RS10450	2259700..2260236	+	537	WP_180924939.1	hypothetical protein	-
H0I86_RS10455	2260233..2260496	+	264	WP_180924940.1	hypothetical protein	-
H0I86_RS10460	2260718..2261479	+	762	WP_180924941.1	hypothetical protein	-
H0I86_RS10465	2261460..2262266	+	807	WP_180924942.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2245238..2323092	77854

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-94)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13208.96 Da
• Isoelectric Point: 9.6391

>T168407 WP_062823026.1 NZ_CP059162:2257449-2257793 [Pseudomonas chlororaphis subsp. aurantiaca]
MKTVFFETTSFTATVGDYLTDDEYRQLQAEMQANPTAGDVMPRTGGFRKLRWHDSRRGKGKRGGLRVIYYWLLDNDQFWM
FSIYDKDEVTKLTSDQEKALKAAIDAELKKRGGK

Nucleotide

• Length: 345 bp

>T168407 NZ_CP075852:4685745-4685996 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12044.07 Da
• Isoelectric Point: 10.0926

>AT168407 WP_124304706.1 NZ_CP059162:2257790-2258104 [Pseudomonas chlororaphis subsp. aurantiaca]
MKKRDLFAELMQGVEEMGEHRKGKITLRQYEMEPLPAPEVTAAEIISIREKLHMSQPVFARQIRTSPDTLKNWEQSKSKP
NAQAALLIKLVQRFPDMVERLNTV

Nucleotide

• Length: 315 bp

>AT168407 NZ_CP075852:4685993-4686247 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A7D6EBU8