Detailed information of TA system TA168362

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 133477..133806
• Replicon: plasmid pEc55_1
• Accession: NZ_CP059131
• Organism: Escherichia coli strain Ec55

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: H0X92_RS26255
• Protein ID: WP_001372321.1
• Coordinates: 133477..133602 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	133679..133806 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0X92_RS26215 (128590)	128590..128817	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
H0X92_RS26220 (128905)	128905..129582	-	678	WP_001348626.1	PAS domain-containing protein	-
H0X92_RS26225 (129716)	129716..130099	-	384	WP_001151566.1	conjugal transfer relaxosome DNA-binding protein TraM	-
H0X92_RS26230 (130429)	130429..131031	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
H0X92_RS26235 (131328)	131328..132149	-	822	WP_001234469.1	DUF932 domain-containing protein	-
H0X92_RS26240 (132268)	132268..132555	-	288	WP_000107535.1	hypothetical protein	-
H0X92_RS26245 (132580)	132580..132786	-	207	WP_000275859.1	hypothetical protein	-
H0X92_RS26780 (132856)	132856..133029	+	174	Protein_157	hypothetical protein	-
H0X92_RS26785 (133027)	133027..133257	-	231	WP_001426396.1	hypothetical protein	-
H0X92_RS26255 (133477)	133477..133602	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
H0X92_RS26790 (133544)	133544..133693	-	150	Protein_160	plasmid maintenance protein Mok	-
- (133679)	133679..133806	-	128	NuclAT_0	-	Antitoxin
- (133679)	133679..133806	-	128	NuclAT_0	-	Antitoxin
- (133679)	133679..133806	-	128	NuclAT_0	-	Antitoxin
- (133679)	133679..133806	-	128	NuclAT_0	-	Antitoxin
- (135248)	135248..135350	-	103	NuclAT_1	-	-
- (135248)	135248..135350	-	103	NuclAT_1	-	-
- (135248)	135248..135350	-	103	NuclAT_1	-	-
- (135248)	135248..135350	-	103	NuclAT_1	-	-
H0X92_RS26265 (135319)	135319..136081	-	763	Protein_162	plasmid SOS inhibition protein A	-
H0X92_RS26270 (136078)	136078..136512	-	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
H0X92_RS26275 (136567)	136567..138525	-	1959	WP_042045562.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15	senB	1..167739	167739

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T168362 WP_001372321.1 NZ_CP059131:c133602-133477 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T168362 NZ_CP075737:c371084-370977 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 128 bp

>AT168362 NZ_CP059131:c133806-133679 [Escherichia coli]
CGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGAAGATAGCCCCGTAGTAA
GTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin