Detailed information of TA system TA168360

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 94210..94449
• Replicon: plasmid pEc55_1
• Accession: NZ_CP059131
• Organism: Escherichia coli strain Ec55

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: H0X92_RS26030
• Protein ID: WP_023144756.1
• Coordinates: 94210..94344 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	94389..94449 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0X92_RS25995 (89912)	89912..90769	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
H0X92_RS26000 (90762)	90762..90836	-	75	WP_031943482.1	RepA leader peptide Tap	-
H0X92_RS26005 (90836)	90836..90967	-	132	Protein_109	protein CopA/IncA	-
H0X92_RS26010 (91073)	91073..91288	-	216	Protein_110	replication regulatory protein RepA	-
H0X92_RS26015 (91479)	91479..93020	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
H0X92_RS26020 (93035)	93035..93781	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
H0X92_RS26030 (94210)	94210..94344	-	135	WP_023144756.1	Hok/Gef family protein	Toxin
- (94389)	94389..94449	+	61	NuclAT_2	-	Antitoxin
- (94389)	94389..94449	+	61	NuclAT_2	-	Antitoxin
- (94389)	94389..94449	+	61	NuclAT_2	-	Antitoxin
- (94389)	94389..94449	+	61	NuclAT_2	-	Antitoxin
H0X92_RS26035 (94416)	94416..94702	-	287	Protein_114	DUF2726 domain-containing protein	-
H0X92_RS26040 (95215)	95215..95427	-	213	WP_013023861.1	hypothetical protein	-
H0X92_RS26045 (95558)	95558..96118	-	561	WP_000139341.1	fertility inhibition protein FinO	-
H0X92_RS26050 (96173)	96173..96919	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15	senB	1..167739	167739

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T168360 WP_023144756.1 NZ_CP059131:c94344-94210 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T168360 NZ_CP075733:32944-33195 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT168360 NZ_CP059131:94389-94449 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin