Detailed information of TA system TA168359

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5186618..5187220
• Replicon: chromosome
• Accession: NZ_CP059130
• Organism: Escherichia coli strain Ec55

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: H0X92_RS25300
• Protein ID: WP_000897302.1
• Coordinates: 5186618..5186929 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H0X92_RS25305
• Protein ID: WP_000356397.1
• Coordinates: 5186930..5187220 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0X92_RS25275 (5182532)	5182532..5183131	+	600	WP_001296610.1	glucose-1-phosphatase	-
H0X92_RS25280 (5183125)	5183125..5183997	+	873	WP_000920762.1	virulence factor BrkB family protein	-
H0X92_RS25285 (5183994)	5183994..5184431	+	438	WP_000560981.1	D-aminoacyl-tRNA deacylase	-
H0X92_RS25290 (5184476)	5184476..5185417	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H0X92_RS25295 (5185481)	5185481..5186389	-	909	WP_001385591.1	alpha/beta hydrolase	-
H0X92_RS25300 (5186618)	5186618..5186929	+	312	WP_000897302.1	hypothetical protein	Toxin
H0X92_RS25305 (5186930)	5186930..5187220	+	291	WP_000356397.1	NadS family protein	Antitoxin
H0X92_RS25310 (5187579)	5187579..5187857	+	279	WP_001296612.1	hypothetical protein	-
H0X92_RS25315 (5188254)	5188254..5188472	+	219	WP_001251293.1	CopG family transcriptional regulator	-
H0X92_RS26765 (5188657)	5188657..5189397	-	741	WP_000608806.1	hypothetical protein	-
H0X92_RS25325 (5189422)	5189422..5190270	-	849	WP_115800220.1	hypothetical protein	-
H0X92_RS25330 (5190560)	5190560..5190802	+	243	WP_001068514.1	CopG family transcriptional regulator	-
H0X92_RS25335 (5190984)	5190984..5191913	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T168359 WP_000897302.1 NZ_CP059130:5186618-5186929 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T168359 NZ_CP075733:1050-1298 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT168359 WP_000356397.1 NZ_CP059130:5186930-5187220 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT168359 NZ_CP075733:1295-1642 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: S1P416