Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4372709..4373508 Replicon chromosome
Accession NZ_CP059130
Organism Escherichia coli strain Ec55

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag H0X92_RS21335 Protein ID WP_000347251.1
Coordinates 4373044..4373508 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag H0X92_RS21330 Protein ID WP_001296435.1
Coordinates 4372709..4373044 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0X92_RS21315 (4368494) 4368494..4369264 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
H0X92_RS21320 (4369280) 4369280..4370614 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
H0X92_RS21325 (4370989) 4370989..4372560 + 1572 WP_001273763.1 galactarate dehydratase -
H0X92_RS21330 (4372709) 4372709..4373044 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H0X92_RS21335 (4373044) 4373044..4373508 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H0X92_RS21340 (4373563) 4373563..4374372 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H0X92_RS21345 (4374621) 4374621..4375901 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
H0X92_RS21350 (4375924) 4375924..4376397 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H0X92_RS21355 (4376408) 4376408..4377187 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
H0X92_RS21360 (4377177) 4377177..4378055 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
H0X92_RS21365 (4378073) 4378073..4378507 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T168357 WP_000347251.1 NZ_CP059130:4373044-4373508 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T168357 NZ_CP075732:c36576-36427 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACTGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT168357 WP_001296435.1 NZ_CP059130:4372709-4373044 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT168357 NZ_CP075732:36620-36681 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References