Detailed information of TA system TA168353

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 3005276..3005778
• Replicon: chromosome
• Accession: NZ_CP059130
• Organism: Escherichia coli strain Ec55

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: H0X92_RS14900
• Protein ID: WP_000767819.1
• Coordinates: 3005276..3005530 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: H0X92_RS14905
• Protein ID: WP_001259253.1
• Coordinates: 3005527..3005778 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0X92_RS14875 (3001094)	3001094..3001552	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
H0X92_RS14880 (3001769)	3001769..3003127	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
H0X92_RS14885 (3003117)	3003117..3003179	-	63	WP_010723108.1	membrane protein YoeI	-
H0X92_RS14890 (3003394)	3003394..3004323	-	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
H0X92_RS14895 (3004369)	3004369..3005193	-	825	WP_000754737.1	SDR family oxidoreductase	-
H0X92_RS14900 (3005276)	3005276..3005530	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
H0X92_RS14905 (3005527)	3005527..3005778	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
H0X92_RS14910 (3006061)	3006061..3006111	+	51	WP_001364200.1	his operon leader peptide	-
H0X92_RS14915 (3006257)	3006257..3007156	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
H0X92_RS14920 (3007162)	3007162..3008466	+	1305	WP_001296211.1	histidinol dehydrogenase	-
H0X92_RS14925 (3008463)	3008463..3009533	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
H0X92_RS14930 (3009533)	3009533..3010600	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3001094..3001552	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T168353 WP_000767819.1 NZ_CP059130:c3005530-3005276 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T168353 NZ_CP075731:c4223641-4223291 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATAACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT168353 WP_001259253.1 NZ_CP059130:c3005778-3005527 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT168353 NZ_CP075731:c4223886-4223635 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGTGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3