Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1293089..1293768 Replicon chromosome
Accession NZ_CP059130
Organism Escherichia coli strain Ec55

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag H0X92_RS06120 Protein ID WP_000057523.1
Coordinates 1293089..1293391 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag H0X92_RS06125 Protein ID WP_000806442.1
Coordinates 1293427..1293768 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0X92_RS06095 (1288465) 1288465..1290117 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
H0X92_RS06100 (1290155) 1290155..1290658 - 504 WP_000667000.1 hypothetical protein -
H0X92_RS06105 (1290655) 1290655..1291311 - 657 WP_015674862.1 hypothetical protein -
H0X92_RS06110 (1291479) 1291479..1291958 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
H0X92_RS06115 (1292162) 1292162..1292956 - 795 WP_000365147.1 TraB/GumN family protein -
H0X92_RS06120 (1293089) 1293089..1293391 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H0X92_RS06125 (1293427) 1293427..1293768 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
H0X92_RS06130 (1293826) 1293826..1296330 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
H0X92_RS06135 (1296592) 1296592..1297524 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T168338 WP_000057523.1 NZ_CP059130:1293089-1293391 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T168338 NZ_CP075731:183749-183856 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAG

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT168338 WP_000806442.1 NZ_CP059130:1293427-1293768 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT168338 NZ_CP075731:c183692-183637 [Escherichia coli]
TCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References