Detailed information of TA system TA168324

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 101239..101472
• Replicon: plasmid pEc45_1
• Accession: NZ_CP059126
• Organism: Escherichia coli strain EC45

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: H0X93_RS24505
• Protein ID: WP_001372321.1
• Coordinates: 101239..101364 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	101441..101472 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0X93_RS24480 (96613)	96613..97302	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
H0X93_RS24485 (97489)	97489..97872	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
H0X93_RS24490 (98193)	98193..98795	+	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
H0X93_RS24495 (99092)	99092..99913	-	822	WP_001234426.1	DUF932 domain-containing protein	-
H0X93_RS24500 (100031)	100031..100318	-	288	WP_000107535.1	hypothetical protein	-
H0X93_RS25195 (100343)	100343..100549	-	207	WP_000547968.1	hypothetical protein	-
H0X93_RS25200 (100619)	100619..100791	+	173	Protein_119	hypothetical protein	-
H0X93_RS25205 (100789)	100789..101019	-	231	WP_071886920.1	hypothetical protein	-
H0X93_RS24505 (101239)	101239..101364	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
H0X93_RS25210 (101306)	101306..101455	-	150	Protein_122	plasmid maintenance protein Mok	-
- (101441)	101441..101472	-	32	NuclAT_1	-	Antitoxin
- (101441)	101441..101472	-	32	NuclAT_1	-	Antitoxin
- (101441)	101441..101472	-	32	NuclAT_1	-	Antitoxin
- (101441)	101441..101472	-	32	NuclAT_1	-	Antitoxin
- (102914)	102914..103111	-	198	NuclAT_0	-	-
- (102914)	102914..103111	-	198	NuclAT_0	-	-
- (102914)	102914..103111	-	198	NuclAT_0	-	-
- (102914)	102914..103111	-	198	NuclAT_0	-	-
H0X93_RS24515 (102923)	102923..103111	+	189	WP_001299721.1	hypothetical protein	-
H0X93_RS24520 (103080)	103080..103842	-	763	Protein_125	plasmid SOS inhibition protein A	-
H0X93_RS24525 (103839)	103839..104273	-	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
H0X93_RS24530 (104328)	104328..104525	-	198	Protein_127	hypothetical protein	-
H0X93_RS24535 (104553)	104553..104786	-	234	WP_000005990.1	DUF905 family protein	-
H0X93_RS24540 (104854)	104854..105351	-	498	WP_042039995.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / aac(3)-IIa / tet(B) / catA1 / mph(A) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..146488	146488

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T168324 WP_001372321.1 NZ_CP059126:c101364-101239 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T168324 NZ_CP075722:1802004-1802258 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Nucleotide

• Length: 32 bp

>AT168324 NZ_CP059126:c101472-101441 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin