Detailed information of TA system TA168320

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 58583..58837
• Replicon: plasmid pEc45_1
• Accession: NZ_CP059126
• Organism: Escherichia coli strain EC45

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: H0X93_RS24260
• Protein ID: WP_001312851.1
• Coordinates: 58583..58732 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	58776..58837 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0X93_RS24230 (53830)	53830..54741	-	912	WP_000440183.1	carbamate kinase	-
H0X93_RS24235 (54752)	54752..55972	-	1221	WP_000410951.1	arginine deiminase	-
H0X93_RS24240 (56679)	56679..57293	+	615	Protein_65	VENN motif pre-toxin domain-containing protein	-
H0X93_RS24245 (57293)	57293..57739	-	447	Protein_66	plasmid replication initiator RepA	-
H0X93_RS24250 (57732)	57732..57806	-	75	WP_032336874.1	RepA leader peptide Tap	-
H0X93_RS24255 (58042)	58042..58299	-	258	WP_000083833.1	replication regulatory protein RepA	-
H0X93_RS24260 (58583)	58583..58732	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (58776)	58776..58837	+	62	NuclAT_2	-	Antitoxin
- (58776)	58776..58837	+	62	NuclAT_2	-	Antitoxin
- (58776)	58776..58837	+	62	NuclAT_2	-	Antitoxin
- (58776)	58776..58837	+	62	NuclAT_2	-	Antitoxin
H0X93_RS24265 (58976)	58976..59158	-	183	WP_000968309.1	hypothetical protein	-
H0X93_RS24270 (59259)	59259..59875	+	617	Protein_71	IS1-like element IS1A family transposase	-
H0X93_RS24275 (59913)	59913..61484	-	1572	WP_023149734.1	IS66-like element ISCro1 family transposase	-
H0X93_RS24280 (61504)	61504..61851	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
H0X93_RS24285 (61851)	61851..62528	-	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
H0X93_RS24290 (62583)	62583..62672	+	90	Protein_75	IS1 family transposase	-
H0X93_RS24295 (62973)	62973..63185	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / aac(3)-IIa / tet(B) / catA1 / mph(A) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..146488	146488

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T168320 WP_001312851.1 NZ_CP059126:c58732-58583 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T168320 NZ_CP075722:843524-843931 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT168320 NZ_CP059126:58776-58837 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin