Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4037317..4038116 Replicon chromosome
Accession NZ_CP059125
Organism Escherichia coli strain EC45

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag H0X93_RS19470 Protein ID WP_000347251.1
Coordinates 4037652..4038116 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H0X93_RS19465 Protein ID WP_001307405.1
Coordinates 4037317..4037652 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0X93_RS19450 (4033102) 4033102..4033872 - 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -
H0X93_RS19455 (4033888) 4033888..4035222 - 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
H0X93_RS19460 (4035597) 4035597..4037168 + 1572 WP_001273761.1 galactarate dehydratase -
H0X93_RS19465 (4037317) 4037317..4037652 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H0X93_RS19470 (4037652) 4037652..4038116 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H0X93_RS19475 (4038171) 4038171..4038980 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H0X93_RS19480 (4039229) 4039229..4040509 + 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
H0X93_RS19485 (4040532) 4040532..4041005 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H0X93_RS19490 (4041016) 4041016..4041795 + 780 WP_219327087.1 PTS N-acetylgalactosamine transporter subunit IIC -
H0X93_RS19495 (4041785) 4041785..4042663 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
H0X93_RS19500 (4042681) 4042681..4043115 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4026442..4038116 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T168316 WP_000347251.1 NZ_CP059125:4037652-4038116 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T168316 NZ_CP075722:184830-184937 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT168316 WP_001307405.1 NZ_CP059125:4037317-4037652 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT168316 NZ_CP075722:c184782-184716 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References